| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM173 |
| Uniprot No | Q86WV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-379aa |
| 氨基酸序列 | MPHSSLHPSIPCPRGHGAQKAALVLLSACLVTLWGLGEPPEHTLRYLVLHLASLQLGLLLNGVCSLAEELRHIHSRYRGSYWRTVRACLGCPLRRGALLLLSIYFYYSLPNAVGPPFTWMLALLGLSQALNILLGLKGLAPAEISAVCEKGNFNVAHGLAWSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS |
| 预测分子量 | 42,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与TMEM173(STING)重组蛋白相关的代表性文献摘要：
1. **文献名称**: *Structural basis of STING binding with and phosphorylation by TBK1*
**作者**: Zhao, B. et al.
**摘要**: 该研究通过冷冻电镜解析了人源STING蛋白与激酶TBK1的复合物结构，揭示了STING激活后招募TBK1进行信号转导的分子机制，为调控先天免疫应答提供了结构基础。
2. **文献名称**: *STING is a direct innate immune sensor of cyclic di-GMP*
**作者**: Burdette, D.L. et al.
**摘要**: 本文首次证明重组STING蛋白可直接结合细菌第二信使c-di-GMP，触发I型干扰素通路，揭示了STING在抗细菌免疫中的核心作用，并阐明了其配体结合特性。
3. **文献名称**: *The cytosolic DNA sensor cGAS forms an oligomeric complex to catalyze STING activation*
**作者**: Li, X. et al.
**摘要**: 研究通过体外重组蛋白实验，发现cGAS在结合DNA后形成多聚体，促进STING的激活及下游信号传导，阐明了cGAS-STING通路中蛋白相互作用的动态过程。
4. **文献名称**: *Anticancer flavonoid analogues induce STING-mediated cytosolic DNA sensing and immune responses*
**作者**: Hong, Z. et al.
**摘要**: 利用重组STING蛋白进行药物筛选，发现类黄酮衍生物可通过直接结合STING激活其活性，为开发新型STING激动剂用于癌症免疫治疗提供了实验依据。
(注：以上文献为领域内关键研究示例，实际引用时请核对具体发表信息。)
TMEM173. also known as STING (Stimulator of Interferon Genes), is a transmembrane protein that plays a critical role in innate immune responses. It acts as a cytosolic DNA sensor, detecting pathogenic or mislocalized self-DNA to initiate downstream signaling cascades. Upon activation, STING recruits TBK1 and IRF3. leading to the production of type I interferons (IFNs) and proinflammatory cytokines. This pathway is essential for antiviral defense, antitumor immunity, and the regulation of autoimmune diseases.
Recombinant TMEM173 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional STING for research and therapeutic development. Its production enables studies on structural dynamics, post-translational modifications, and interaction networks. Researchers utilize recombinant STING to investigate mechanisms of immune activation, screen agonists/antagonists, and design immunotherapies.
In cancer therapy, STING agonists are explored to enhance antitumor immunity by activating dendritic cells and T cells. Conversely, inhibiting STING may mitigate autoimmune conditions driven by aberrant DNA sensing. Structural insights from recombinant protein studies (e.g., cryo-EM resolved STING conformations) have guided drug design, including small molecules and cyclic dinucleotides. Challenges remain in optimizing stability, delivery, and minimizing off-target effects.
Overall, recombinant TMEM173 serves as a pivotal tool for decoding immune regulation and advancing precision immunotherapies, bridging molecular biology with clinical applications.
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