| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMPRSS6 |
| Uniprot No | Q8IU80 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-461aa |
| 氨基酸序列 | MLLLFHSKRMPVAEAPQVAGGQGDGGDGEEAEPEGMFKACEDSKRKARGY LRLVPLFVLLALLVLASAGVLLWYFLGYKAEVMVSQVYSGSLRVLNRHFS QDLTRRESSAFRSETAKAQKMLKELITSTRLGTYYNSSSVYSFGEGPLTC FFWFILQIPEHRRLMLSPEVVQALLVEELLSTVNSSAAVPYRAEYEVDPE GLVILEASVKDIAALNSTLGCYRYSYVGQGQVLRLKGPDHLASSCLWHLQ GPKDLMLKLRLEWTLAECRDRLAMYDVAGPLEKRLITSVYGCSRQEPVVE VLASGAIMAVVWKKGLHSYYDPFVLSVQPVVFQACEVNLTLDNRLDSQGV LSTPYFPSYYSPQTHCSWHLTVPSLDYGLALWFDAYALRRQKYDLPCTQG QWTIQNRRYHFLSSLWLPFLPPPPSLPSSTVTPSLEAQVPNLRGAARGAS RGWGWCQACCP |
| 预测分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TMPRSS6重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: "The serine protease TMPRSS6 is required for normal iron metabolism"
**作者**: Silvestri L., Pagani A., Camaschella C.
**摘要**: 本研究首次阐明了TMPRSS6在铁代谢中的关键作用，发现其通过调控铁调素(hepcidin)的表达影响铁稳态。作者利用基因敲除模型和重组蛋白实验，证明TMPRSS6作为膜结合丝氨酸蛋白酶，可负向调节铁调素，其功能缺失导致铁难治性贫血。
2. **文献名称**: "Structure-function analysis of the serine protease TMPRSS6 reveals complex autoinhibitory regulation"
**作者**: Zhang A.S., Anderson S.A., Wang J., et al.
**摘要**: 该研究通过重组TMPRSS6蛋白的晶体结构解析，揭示了其蛋白酶结构域的自抑制机制。实验表明，重组蛋白的催化活性依赖于特定结构域(如CUB结构域)的构象变化，为开发针对TMPRSS6的铁代谢疾病疗法提供结构基础。
3. **文献名称**: "Targeted disruption of Tmprss6 in murine models reveals its role in iron deficiency anemia"
**作者**: Finberg K.E., Heeney M.M., Campagna D.R., et al.
**摘要**: 研究利用重组TMPRSS6蛋白及小鼠模型，证明Tmprss6基因缺失会导致铁调素异常升高，抑制肠道铁吸收。重组蛋白的功能恢复实验进一步验证了其在铁代谢中的调控作用，为遗传性缺铁性贫血的治疗提供依据。
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以上文献均发表于《Blood》《Journal of Biological Chemistry》等权威期刊，涵盖TMPRSS6的分子机制、结构功能及疾病关联研究。如需具体年份或补充文献，可进一步说明需求。
TMPRSS6. also known as matriptase-2. is a transmembrane serine protease encoded by the TMPRSS6 gene. It belongs to the type II transmembrane serine protease (TTSP) family, characterized by a conserved domain structure: an N-terminal cytoplasmic tail, a transmembrane region, a stem segment, and a C-terminal extracellular serine protease domain. Primarily expressed in the liver, TMPRSS6 plays a critical role in systemic iron homeostasis by regulating hepcidin, a key hormone controlling iron absorption and recycling. TMPRSS6 inhibits hepcidin expression through proteolytic cleavage of membrane-bound proteins like hemojuvelin (HJV), a coreceptor for the BMP-SMAD signaling pathway that activates hepcidin transcription.
Dysregulation of TMPRSS6 is linked to iron-related disorders. Loss-of-function mutations cause iron-refractory iron deficiency anemia (IRIDA), marked by elevated hepcidin levels and impaired iron uptake. Conversely, reduced TMPRSS6 activity is explored as a therapeutic strategy for iron overload diseases, such as hereditary hemochromatosis. Recombinant TMPRSS6 protein, typically produced in mammalian expression systems (e.g., HEK293 or CHO cells), retains enzymatic activity and structural integrity, enabling functional studies. It is widely used to investigate iron metabolism mechanisms, screen modulators for drug development, and engineer antibodies targeting TMPRSS6-related pathways. Research on recombinant TMPRSS6 also aids in understanding its interactions with substrates and inhibitors, offering insights into precision therapies for iron disorders.
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