| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CX40 |
| Uniprot No | P36382 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MGDWSFLGNFLEEVHKHSTVVGKVWLTVLFIFRMLVLGTAAESSWGDEQADFRCDTIQPGCQNVCYDQAFPISHIRYWVLQIIFVSTPSLVYMGHAMHTVRMQEKRKLREAERAKEVRGSGSYEYPVAEKAELSCWEEGNGRIALQGTLLNTYVCSILIRTTMEVGFIVGQYFIYGIFLTTLHVCRRSPCPHPVNCYVSRPTEKNVFIVFMLAVAALSLLLSLAELYHLGWKKIRQRFVKPRQHMAKCQLSGPSVGIVQSCTPPPDFNQCLENGPGGKFFNPFSNNMASQQNTDNLVTEQVRGQEQTPGEGFIQVRYGQKPEVPNGVSPGHRLPHGYHSDKRRLSKASSKARSDDLSV |
| 预测分子量 | 41.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CX40(Connexin 40)重组蛋白的3篇参考文献示例(注：内容为虚构，仅作格式参考)：
1. **文献名称**：*Expression and Purification of Recombinant Connexin40 in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：研究报道了通过大肠杆菌表达系统成功克隆并纯化CX40重组蛋白，优化了可溶性表达条件，并通过Western blot验证其抗原性。
2. **文献名称**：*Functional Characterization of Recombinant CX40 Gap Junction Channels in vitro*
**作者**：Wang L, et al.
**摘要**：利用脂质体双层技术分析重组CX40形成的间隙连接通道电导特性，发现其具有电压依赖性门控，并参与细胞间信号传递调控。
3. **文献名称**：*Crystallographic Analysis of Recombinant CX40 Hemichannel Structure*
**作者**：Garcia R, et al.
**摘要**：首次解析了重组CX40蛋白的高分辨率晶体结构，揭示了其跨膜结构域的关键氨基酸位点，为靶向药物设计提供依据。
4. **文献名称**：*Recombinant CX40 Protein Attenuates Cardiac Arrhythmia in Mouse Models*
**作者**：Chen H, et al.
**摘要**：动物实验表明，外源性重组CX40蛋白可通过增强心肌细胞间耦合改善心律失常，提示其潜在治疗价值。
(注：以上文献为模拟生成，实际引用需以真实数据库为准。)
CX40 recombinant protein, derived from the connexin 40 (Cx40) gene, is a key component in studying intercellular communication mechanisms. Connexins are transmembrane proteins that assemble into hexameric structures called connexons, forming gap junctions. These channels facilitate direct exchange of ions, metabolites, and signaling molecules between adjacent cells. Cx40 is predominantly expressed in vascular endothelial cells, atrial cardiomyocytes, and specific smooth muscle tissues, playing critical roles in cardiovascular homeostasis, electrical conduction, and vascular tone regulation.
The recombinant form is produced using genetic engineering techniques, typically by inserting the human or murine Cx40 gene into bacterial, insect, or mammalian expression systems. Post-translational modifications in eukaryotic systems ensure proper folding and functionality. Purification methods often involve affinity tags (e.g., His-tag) and chromatography. Recombinant CX40 retains the protein's structural features, including four transmembrane domains, two extracellular loops, and cytoplasmic N-/C-termini, enabling its use in functional studies.
Research applications focus on elucidating Cx40's role in cardiovascular pathologies. Mutations or dysregulation of Cx40 are linked to atrial fibrillation, hypertension, and atherosclerosis. The recombinant protein aids in characterizing gap junction assembly, conductance properties, and interactions with partners like Cx37 or Cx43. It also serves as an antigen for antibody development and a tool for drug screening targeting connexin-related diseases. Additionally, it supports tissue engineering by mimicking native cell-cell communication in synthetic constructs. Studies using CX40 recombinant protein have advanced understanding of vascular remodeling and arrhythmia mechanisms, highlighting its therapeutic potential in cardiovascular regeneration and precision medicine.
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