| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAMK2g |
| Uniprot No | Q13555 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-527aa |
| 氨基酸序列 | MATTATCTRFTDDYQLFEELGKGAFSGVRRCVKKTPTQEYAAKIINTKKL SARDHQKLEREARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFE DIVAREYYSEADASHCIHQILESVNHIHQHDIVHRDLKPENLLLASKCKG AAVKLADFGLAIEVQGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDIWAC GVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLIN QMLTINPAKRITADQALKHPWVCQRSTVASMMHRQETVECLRKFNARRKL KGAILTTMLVSRNFSVGRQSSAPASPAASAAGLAGQAAKSLLNKKSDGGV KKRKSSSSVHLMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKVRKQEII KITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVEGMDFHKFYFEN LLSKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEE TRVWHRRDGKWLNVHYHCSGAPAAPLQ |
| 预测分子量 | 84 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **CAMK2γ(Ca²+/钙调蛋白依赖性蛋白激酶 II γ)重组蛋白** 的参考文献示例，包含文献名称、作者及简要摘要概括：
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1. **文献名称**：*Structural insights into the regulation of Ca²+/calmodulin-dependent protein kinase IIγ (CAMK2G) by autophosphorylation*
**作者**：Rosenberg, O.S. et al.
**摘要**：通过X射线晶体学解析CAMK2γ自磷酸化前后的结构变化，揭示其激活与自我调控机制，重组蛋白在大肠杆菌中表达并用于功能验证。
2. **文献名称**：*CAMK2G variants link cardiac arrhythmia susceptibility to neuronal calcium signaling*
**作者**：Hoch, B. et al.
**摘要**：研究CAMK2γ基因突变对心脏和神经元钙信号的影响，利用重组蛋白体外实验证明突变体激酶活性异常与心律失常的关联。
3. **文献名称**：*Recombinant CAMK2G as a tool for kinase activity screening in neurodegenerative diseases*
**作者**：Baucum, A.J. et al.
**摘要**：开发基于CAMK2γ重组蛋白的高通量激酶活性检测体系，用于筛选阿尔茨海默病相关tau蛋白磷酸化的潜在抑制剂。
4. **文献名称**：*CAMK2G-mediated synaptic plasticity and memory formation requires oligomerization*
**作者**：Lledo, P.M. et al.
**摘要**：通过重组CAMK2γ蛋白的体外寡聚实验，结合小鼠模型证明其多聚化对突触可塑性和记忆巩固的关键作用。
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**注**：以上为模拟参考文献，实际文献需通过数据库(如PubMed、Google Scholar)检索确认。建议使用关键词 **“CAMK2G recombinant protein”** 或 **“CAMKII gamma kinase activity”** 进一步查找。
CAMK2γ (calcium/calmodulin-dependent protein kinase II gamma) is a member of the CAMKII family, a class of serine/threonine kinases critical for calcium-mediated signaling in eukaryotic cells. Encoded by the CAMK2G gene, the γ isoform is widely expressed, particularly in the brain, heart, and immune cells. CAMKII proteins function as multimeric holoenzymes, with each subunit containing a catalytic domain, a regulatory domain (with autoinhibitory and calmodulin-binding regions), and a variable domain that determines subcellular targeting. CAMK2γ is activated by calcium/calmodulin binding, triggering autophosphorylation at Thr287. which sustains kinase activity even after calcium levels drop—a feature enabling signal amplification and long-term cellular responses.
Recombinant CAMK2γ proteins are engineered for research and therapeutic applications. Produced via heterologous expression systems (e.g., E. coli, insect, or mammalian cells), these proteins retain kinase activity and structural integrity for functional studies. Purification typically involves affinity tags (e.g., His-tag) followed by ion-exchange chromatography. Recombinant CAMK2γ is used to investigate calcium signaling pathways, synaptic plasticity, cardiac hypertrophy, and immune cell activation. Its dysregulation is implicated in neurological disorders (e.g., Alzheimer’s), arrhythmias, and cancer progression, making it a target for drug discovery. In vitro, it serves as a tool for kinase assays, substrate phosphorylation studies, and inhibitor screening. Structural analyses of recombinant CAMK2γ have clarified mechanisms of autoinhibition, activation, and isoform-specific interactions. Despite functional overlaps with other CAMKII isoforms (α, β, δ), γ-specific roles in nuclear signaling and gene regulation highlight its unique biological significance. Ongoing research leverages recombinant CAMK2γ to dissect its pathological contributions and develop isoform-selective therapeutics.
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