| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HCAM |
| Uniprot No | P54750 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-535aa |
| 氨基酸序列 | MGSSATEIEELENTTFKYLTGEQTEKMWQRLKGILRCLVKQLERGDVNVVDLKKNIEYAASVLEAVYIDETRRLLDTEDELSDIQTDSVPSEVRDWLASTFTRKMGMTKKKPEEKPKFRSIVHAVQAGIFVERMYRKTYHMVGLAYPAAVIVTLKDVDKWSFDVFALNEASGEHSLKFMIYELFTRYDLINRFKIPVSCLITFAEALEVGYSKYKNPYHNLIHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYRLMQEEEMNILINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPEGIDRAKTMSLILHAADISHPAKSWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIVIPLIEEASKAETSSYVASSSTTIVGLHIADALRRSNTKGSMSDGSYSPDYSLAAVDLKSFKNNLVDIIQQNKERWKELAAQEARTSSQKCEFIHQ |
| 预测分子量 | 61,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HCAM(CD44)重组蛋白的3篇模拟参考文献示例，供参考：
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1. **文献名称**：*Expression and Purification of Recombinant Human CD44 in Escherichia coli for Functional Studies*
**作者**：Smith A, et al.
**摘要**：本研究报道了利用大肠杆菌表达系统高效表达可溶性HCAM(CD44)重组蛋白的优化策略，通过His标签纯化获得高纯度蛋白，并验证其在体外与透明质酸结合的能力。
2. **文献名称**：*Structural Analysis of Recombinant CD44v6 Reveals Its Role in Cancer Cell Migration*
**作者**：Li Y, et al.
**摘要**：通过哺乳动物细胞系统表达HCAM变体CD44v6重组蛋白，解析其晶体结构，证明其与细胞外基质相互作用的关键结构域，并揭示其在肿瘤转移中的调控机制。
3. **文献名称**：*Recombinant HCAM Protein as a Targeted Drug Delivery Carrier in Inflammatory Diseases*
**作者**：Wang X, et al.
**摘要**：开发了一种基于重组HCAM蛋白的药物递送系统，通过体外和动物模型验证其靶向炎症部位的能力，为治疗类风湿性关节炎提供潜在策略。
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注：以上文献为示例，实际引用时需以真实发表的论文为准。建议通过PubMed或Web of Science等数据库检索关键词(如“recombinant CD44 protein”“HCAM expression”)获取具体文献。
HCAM (Homing Cell Adhesion Molecule), also known as CD44. is a transmembrane glycoprotein that plays a critical role in cell-cell interactions, cell migration, and signaling. It is widely expressed in various cell types, including immune cells, stem cells, and cancer cells. HCAM interacts with extracellular matrix components, particularly hyaluronic acid (HA), mediating cell adhesion, inflammation, and tumor metastasis. Its isoforms, generated by alternative splicing, exhibit functional diversity in tissue homeostasis and disease progression.
Recombinant HCAM proteins are engineered using biotechnological platforms (e.g., bacterial, mammalian, or insect cell systems) to study its structure-function relationships or therapeutic potential. These proteins retain key domains, such as the HA-binding region, enabling researchers to investigate HCAM’s role in pathological processes like cancer invasion, immune regulation, and wound healing. In oncology, HCAM is linked to cancer stem cell maintenance and chemoresistance, making recombinant variants valuable for drug screening or targeted therapy development.
The production of recombinant HCAM often involves codon optimization and affinity tag fusion (e.g., His-tag) to enhance solubility and purification efficiency. Post-translational modifications, such as glycosylation, are preserved in eukaryotic expression systems to mimic native protein behavior. Applications include in vitro binding assays, inhibitor studies, and biomarker validation. Recent advances also explore HCAM-based biologics for modulating immune responses or delivering therapeutics to HA-rich microenvironments.
Despite challenges in structural complexity, recombinant HCAM remains a pivotal tool for decoding its multifaceted roles and translating findings into clinical interventions.
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