| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABP1 |
| Uniprot No | P19801 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-751aa |
| 氨基酸序列 | MPALGWAVAA ILMLQTAMAE PSPGTLPRKA GVFSDLSNQE LKAVHSFLWS KKELRLQPSS TTTMAKNTV FLIEMLLPKK YHVLRFLDKG ERHPVREARA VIFFGDQEHP NVTEFAVGPL PGPCYMRAL SPRPGYQSSW ASRPISTAEY ALLYHTLQEA TKPLHQFFLN TTGFSFQDCH DRCLAFTDV APRGVASGQR RSWLIIQRYV EGYFLHPTGL ELLVDHGSTD AGHWAVEQVW YNGKFYGSP EELARKYADG EVDVVVLEDP LPGGKGHDST EEPPLFSSHK PRGDFPSPIH VSGPRLVQP HGPRFRLEGN AVLYGGWSFA FRLRSSSGLQ VLNVHFGGER IAYEVSVQEA VALYGGHTP AGMQTKYLDV GWGLGSVTHE LAPGIDCPET ATFLDTFHYY DADDPVHYPR ALCLFEMPT GVPLRRHFNS NFKGGFNFYA GLKGQVLVLR TTSTVYNYDY IWDFIFYPNG VMEAKMHAT GYVHATFYTP EGLRHGTRLH THLIGNIHTH LVHYRVDLDV AGTKNSFQTL QMKLENITN PWSPRHRVVQ PTLEQTQYSW ERQAAFRFKR KLPKYLLFTS PQENPWGHKR TYRLQIHSM ADQVLPPGWQ EEQAITWARY PLAVTKYRES ELCSSSIYHQ NDPWHPPVVF EQFLHNNENI ENEDLVAWV TVGFLHIPHS EDIPNTATPG NSVGFLLRPF NFFPEDPSLA SRDTVIVWPR DNGPNYVQR WIPEDRDCSM PPPFSYNGTY RPV |
| 预测分子量 | 85 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABP1重组蛋白的参考文献及其摘要概括：
1. **《Auxin Binding Protein 1: Functional Insights from Structural Studies》**
- **作者**：Napier, R.M. 等
- **摘要**：该研究解析了ABP1的晶体结构，证明其通过重组蛋白表达保留了与生长素(如IAA)结合的能力，揭示了其在植物细胞信号传导中的潜在作用机制。
2. **《Recombinant ABP1 Mediates Auxin-Dependent Cell Expansion in Mammalian Cells》**
- **作者**：Braun, N. 等
- **摘要**：通过在大肠杆菌中表达重组ABP1蛋白，发现其可在非植物细胞中诱导生长素依赖的细胞膜扩张，支持ABP1在细胞形态调控中的跨物种功能。
3. **《ABP1 Recombinant Protein Enhances Rice Root Development under Stress》**
- **作者**：Dai, X. 等
- **摘要**：利用重组ABP1蛋白处理水稻根系，发现其通过激活生长素响应基因(如AUX/IAA)显著提高植物在盐胁迫环境中的适应性。
4. **《Role of Recombinant ABP1 in Arabidopsis Thaliana Polar Auxin Transport》**
- **作者**：Robert, S. 等
- **摘要**：通过体外重组ABP1蛋白实验，证明其与PIN蛋白互作调控生长素极性运输，影响拟南芥根尖细胞的发育过程。
(注：上述文献为示例，实际引用时需核对具体文献来源。)
ABP1 (Auxin-Binding Protein 1) is a well-characterized plant protein implicated in auxin-mediated signaling pathways, which regulate critical growth and developmental processes such as cell elongation, division, and tropic responses. First identified in the 1980s in maize (Zea mays) coleoptiles, ABP1 was initially proposed as a primary receptor for the plant hormone auxin (e.g., indole-3-acetic acid, IAA). Structurally, it is a ~22 kDa glycoprotein containing an N-terminal signal peptide, a conserved auxin-binding domain, and C-terminal endoplasmic reticulum (ER) retention motifs. However, studies later revealed its localization not only in the ER but also on the plasma membrane, suggesting dual roles in intracellular and extracellular auxin sensing.
Recombinant ABP1 is typically produced using heterologous expression systems (e.g., Escherichia coli or yeast) to study its biochemical properties and interaction mechanisms. Purified recombinant ABP1 retains auxin-binding activity, enabling structural analyses like X-ray crystallography, which revealed its auxin-binding pocket and conformational changes upon ligand binding. Functional studies using recombinant ABP1 have highlighted its potential to activate downstream signaling, including Rho-like GTPase-mediated membrane polarization and proton pump activation, which drive cell expansion.
Despite its historical significance, ABP1's role as a canonical auxin receptor remains controversial. Genetic knockout studies in Arabidopsis showed weak phenotypes, suggesting functional redundancy or alternative auxin perception mechanisms (e.g., TIR1/AFB receptors). Nevertheless, recombinant ABP1 remains a valuable tool for dissecting auxin signaling complexity and engineering crops with optimized growth traits. Recent work also explores its interplay with other auxin transporters and receptors, revitalizing interest in its context-specific roles in plant development.
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