| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGa2 |
| Uniprot No | P17301 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 188-365aa |
| 氨基酸序列 | WDAVKNFLEKFVQGLDIGPTKTQVGLIQYANNPRVVFNLNTYKTKEEMIVATSQTSQYGGDLTNTFGAIQYARKYAYSAASGGRRSATKVMVVVTDGESHDGSMLKAVIDQCNHDNILRFGIAVLGYLNRNALDTKNLIKEIKAIASIPTERYFFNVSDEAALLEKAGTLGEQIFSIE |
| 预测分子量 | 25.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITGa2重组蛋白的参考文献示例(注：内容为示例性概括，实际文献需通过学术数据库验证)：
---
1. **文献名称**: "Recombinant expression and functional characterization of integrin α2 subunit in platelet adhesion"
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组ITGa2蛋白，证实其在血小板胶原结合中的关键作用，为研究血栓形成提供了实验工具。
2. **文献名称**: "Structural insights into the integrin α2β1-collagen interaction through cryo-EM analysis"
**作者**: Tanaka K, et al.
**摘要**: 通过冷冻电镜解析了重组ITGa2与胶原的复合物结构，揭示了其特异性结合的分子机制，为靶向药物设计奠定基础。
3. **文献名称**: "Role of recombinant ITGa2 in cancer cell invasion and metastasis"
**作者**: Chen L, et al.
**摘要**: 利用重组ITGa2蛋白发现其通过增强肿瘤细胞与细胞外基质的粘附促进转移，提示其作为癌症治疗潜在靶点。
4. **文献名称**: "Optimization of mammalian cell expression system for high-yield ITGa2 production"
**作者**: Müller R, et al.
**摘要**: 开发了一种哺乳动物细胞表达系统，显著提高重组ITGa2的产量和活性，解决了传统原核表达中蛋白折叠不足的问题。
---
建议通过PubMed、Web of Science或Google Scholar检索上述关键词，以获取真实文献信息及全文。
**Background of ITGa2 Recombinant Protein**
Integrin alpha-2 (ITGA2), a key subunit of the α2β1 integrin receptor, is a cell surface adhesion molecule involved in mediating interactions between cells and the extracellular matrix (ECM). It plays a critical role in processes such as cell adhesion, migration, signaling, and tissue repair by binding to collagen and laminin within the ECM. The α2 subunit contains a conserved I-domain responsible for ligand recognition, while its β1 partner facilitates signal transduction. Dysregulation of ITGA2 has been linked to pathologies including cancer metastasis, thrombosis, and inflammatory disorders, highlighting its therapeutic and diagnostic potential.
Recombinant ITGa2 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce a purified, functional form of the α2 subunit. This allows researchers to study its structure, ligand-binding mechanisms, and role in cellular pathways without interference from native cellular components. Recombinant variants may include tags (e.g., Fc or His-tags) for easier detection, purification, or immobilization in assays.
Studies utilizing ITGa2 recombinant protein have advanced understanding of collagen receptor biology, platelet activation, and tumor cell invasion. It is also employed in drug screening, antibody development, and biomaterial design for regenerative medicine. By providing a controlled, scalable source of the protein, recombinant ITGA2 supports both basic research and translational applications, bridging gaps between molecular insights and clinical interventions.
×
