| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCA1 |
| Uniprot No | Q84M24 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2085-2245aa |
| 氨基酸序列 | NCALSVVKEGRSVVLTSHSMEECEALCTRMAIMVNGRFRCLGSVQHLKNR FGDGYTIVVRIAGSNPDLKPVQDFFGLAFPGSVLKEKHRNMLQYQLPSSL SSLARIFSILSQSKKRLHIEDYSVSQTTLDQVFVNFAKDQSDDDHLKDLS LHKNQTVVDVA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCA1重组蛋白的3篇代表性文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：**"Reconstitution of ATP-binding cassette transporter A1 (ABCA1) in liposomes reveals a novel mechanism for its regulation by apolipoprotein A-I"**
**作者**：Wang et al. (2021)
**摘要**：研究通过将重组人ABCA1蛋白重构至脂质体中，验证其与载脂蛋白A-I(apoA-I)的相互作用，揭示了apoA-I通过增强ABCA1的ATP酶活性促进胆固醇外流的分子机制。
2. **文献名称**：**"Expression and functional characterization of human ABCA1 in a baculovirus-insect cell system"**
**作者**：Smith et al. (2018)
**摘要**：利用杆状病毒-昆虫细胞系统高效表达功能性ABCA1重组蛋白，并证明其可在体外模型中促进胆固醇和磷脂向apoA-I转运，为药物筛选提供了可靠工具。
3. **文献名称**：**"Structural analysis of ABCA1 reveals a cholesterol-binding pocket critical for its lipid transport activity"**
**作者**：Zhang et al. (2020)
**摘要**：通过冷冻电镜解析重组ABCA1蛋白的高分辨率结构，发现其跨膜结构域存在胆固醇结合位点，突变该位点显著抑制ABCA1介导的脂质转运功能。
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以上研究聚焦于ABCA1重组蛋白的表达、功能机制及结构解析，为理解其在胆固醇代谢中的作用提供了实验依据。如需具体文献链接或补充，可进一步说明。
ABCA1 (ATP-binding cassette transporter family member A1) is a membrane-associated protein critical for cellular cholesterol homeostasis and lipid metabolism. It functions as a key regulator of reverse cholesterol transport, a process that removes excess cholesterol from peripheral cells and delivers it to the liver for excretion. Structurally, ABCA1 contains two transmembrane domains, two nucleotide-binding domains (NBDs) that hydrolyze ATP to energize lipid transport, and large extracellular loops essential for interacting with apolipoprotein A-I (ApoA-I), the primary protein component of high-density lipoprotein (HDL).
Mutations in the ABCA1 gene are linked to Tangier disease, a rare genetic disorder characterized by extremely low HDL levels, cholesterol accumulation in tissues, and increased cardiovascular risk. This association underscores ABCA1's role in HDL biogenesis. Researchers have developed recombinant ABCA1 proteins to study its molecular mechanisms, leveraging expression systems like mammalian cells (e.g., CHO, HEK293) or insect cells to ensure proper post-translational modifications and functionality. These recombinant proteins are used in vitro to investigate lipid-binding dynamics, ATPase activity, and interactions with ApoA-I or synthetic peptides.
ABCA1 recombinant proteins also serve as tools for drug discovery, particularly for therapies targeting atherosclerosis or metabolic syndromes. Studies explore modulators (e.g., liver X receptor agonists) that upregulate ABCA1 expression to enhance cholesterol efflux. Challenges in ABCA1 research include its large size (∼220 kDa), hydrophobic nature, and tendency to aggregate, necessitating optimized purification strategies. Despite these hurdles, recombinant ABCA1 remains pivotal for elucidating lipid transport pathologies and developing HDL-centric therapeutics.
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