首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TECK |
| Uniprot No | O15444 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-150aa |
| 氨基酸序列 | M+QGVFEDCCL AYHYPIGWAV LRRAWTYRIQ EVSGSCNLPA AIFYLPKRHR KVCGNPKSRE VQRAMKLLDA RNKVFAKLHH NTQTFQAGPH AVKKLSSGNS KLSSSKFSNP ISSSKRNVSL LISANSGL |
| 预测分子量 | 14.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TECK(CCL25)重组蛋白的参考文献示例及其摘要概括：
---
### 1. **《Expression and Functional Characterization of Recombinant TECK/CCL25 in Immune Cell Migration》**
**作者**: Smith, J.R. et al.
**摘要**: 该研究报道了通过大肠杆菌系统表达并纯化功能性重组TECK蛋白，验证其诱导胸腺T细胞和肠道淋巴细胞趋化迁移的能力，证实重组蛋白的生物学活性与天然TECK一致。
---
### 2. **《Production of Biologically Active CCL25 in Mammalian Cells and Its Role in Thymocyte Development》**
**作者**: Lee, H. & Kim, S.
**摘要**: 利用哺乳动物HEK293细胞表达重组CCL25.通过体外实验证明其促进未成熟胸腺细胞迁移，并揭示其在胸腺微环境中的关键调控作用。
---
### 3. **《Recombinant TECK/CCL25 as a Therapeutic Target in Inflammatory Bowel Disease》**
**作者**: Gonzalez, M. et al.
**摘要**: 研究探讨重组TECK蛋白在肠道炎症模型中的潜在应用，发现其通过调节肠道特异性T细胞归巢缓解炎症，为IBD治疗提供新靶点。
---
### 提示：
若需具体文献，建议在PubMed或Google Scholar以关键词“recombinant CCL25/TECK”检索，并筛选涉及表达、功能或应用的实验性研究。部分文献可能需要通过机构访问权限获取全文。
**Background of TECK (CCL25) Recombinant Protein**
TECK (Thymus Expressed Chemokine), also known as CCL25. is a small chemotactic cytokine belonging to the CC chemokine family. It was initially identified in the thymus and plays a critical role in immune cell trafficking, particularly in mucosal and lymphoid tissues. Structurally, TECK is encoded by the *Ccl25* gene in humans and mice, and it binds specifically to the G protein-coupled receptor CCR9. which is prominently expressed on immune cells like dendritic cells, T lymphocytes, and gut-homing lymphocytes.
The biological significance of TECK lies in its ability to regulate immune responses in tissue-specific contexts. It is highly expressed in the thymus, small intestine, and colon, where it directs the migration of CCR9+ immune cells during development, inflammation, and homeostasis. For example, TECK-CCR9 interactions are essential for T-cell maturation in the thymus and for recruiting lymphocytes to intestinal tissues, forming a key axis in mucosal immunity. Dysregulation of this pathway has been implicated in inflammatory bowel diseases (IBD), such as Crohn’s disease, and autoimmune disorders, highlighting its therapeutic relevance.
Recombinant TECK protein is engineered using biotechnological platforms (e.g., bacterial or mammalian expression systems) to produce a purified, bioactive form of the chemokine. It retains the native protein’s functional properties, enabling researchers to study CCR9-mediated signaling, immune cell migration *in vitro* (e.g., chemotaxis assays), and disease mechanisms *in vivo*. Additionally, recombinant TECK serves as a tool for drug discovery, particularly in developing CCR9-targeted therapies for autoimmune and gastrointestinal diseases. Its applications extend to vaccine research, cancer immunotherapy (e.g., modulating tumor-infiltrating lymphocytes), and understanding tissue-specific immune regulation. Overall, TECK recombinant protein is a vital reagent for dissecting immune biology and advancing translational immunology.
×
