| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PACAP |
| Uniprot No | P41586 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-120aa |
| 氨基酸序列 | MHSDCIFKKEQAMCLEKIQRANELMGFNDSSPGCPGMWDNITCWKPAHVG EMVLVSCPELFRIFNPDQVWETETIGESDFGDSNSLDLSDMGVVSRNCTE |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PACAP重组蛋白的参考文献及其摘要概括：
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1. **文献名称**: "Expression and functional characterization of recombinant pituitary adenylate cyclase-activating polypeptide (PACAP) in Escherichia coli"
**作者**: Lee JH, et al.
**摘要**: 该研究报道了在大肠杆菌中高效表达重组PACAP38的方法，通过融合蛋白策略提高可溶性表达，并验证其体外激活腺苷酸环化酶的生物活性，为大规模生产功能性PACAP提供技术基础。
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2. **文献名称**: "Neuroprotective effects of recombinant PACAP27 in a mouse model of Parkinson's disease"
**作者**: Wang Y, et al.
**摘要**: 研究利用重组PACAP27在小鼠帕金森模型中验证其神经保护作用，发现其通过抑制线粒体凋亡通路和减少氧化应激，显著改善多巴胺能神经元存活率。
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3. **文献名称**: "Recombinant PACAP enhances glucose-stimulated insulin secretion via PAC1 receptor in pancreatic β-cells"
**作者**: Suzuki K, et al.
**摘要**: 该文献证明重组PACAP通过结合胰岛β细胞的PAC1受体，激活cAMP/PKA信号通路，增强葡萄糖刺激的胰岛素分泌，提示其在糖尿病治疗中的潜在应用价值。
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(注：以上文献信息为模拟示例，实际引用需以具体论文数据为准。)
**Background of PACAP Recombinant Protein**
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a multifunctional neuropeptide belonging to the vasoactive intestinal peptide (VIP)/secretin/glucagon family. It exists in two bioactive forms, PACAP38 and PACAP27. derived from the same precursor through post-translational processing. PACAP38. the predominant isoform, is highly conserved across species and plays critical roles in modulating neurotransmission, hormone secretion, immune responses, and cellular survival. It exerts its effects by binding to G protein-coupled receptors (PAC1. VPAC1. VPAC2), activating cAMP and other signaling pathways.
Recombinant PACAP proteins are engineered using biotechnological platforms (e.g., *E. coli* or mammalian cell systems*) to mimic the native peptide’s structure and function. These proteins are produced via gene cloning, expression, and purification processes, ensuring high purity and bioactivity. Their production enables precise study of PACAP’s mechanisms in physiological and pathological contexts, such as neuroprotection, metabolic regulation, and inflammation.
PACAP recombinant proteins are vital tools in neuroscience, endocrinology, and drug development. Research highlights their potential in treating neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), stroke, diabetes, and traumatic injuries. Additionally, they aid in exploring PACAP’s role in circadian rhythms, stress responses, and gastrointestinal functions. The availability of recombinant PACAP ensures consistency in experimental studies, overcoming challenges associated with isolating the peptide from natural sources.
In summary, PACAP recombinant proteins bridge basic research and therapeutic innovation, offering insights into a peptide central to numerous biological processes while paving the way for clinical applications.
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