| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCB1 |
| Uniprot No | Q9NQ66 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1216aa |
| 氨基酸序列 | MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGRHAKAPKDPKLRELLDVGNIGRLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNNSLARKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSMFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTMDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPTLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSEAPSEARTTPAENGVNHTTTLTPKPPSQALHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTDLIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEPSSPDHGSSTIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHKEIRQQILDEKPKLQVELEQEYQDKFKRLPLEILEFVQEAMKGKISEDSNHGSAPLSLSSDPGKVNHKTPSSEELGGDIPGKEFDTPL |
| 预测分子量 | 138,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLCB1重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**: *"Recombinant expression and functional characterization of human phospholipase C-β1 in Escherichia coli"*
**作者**: Smith A, et al.
**摘要**: 该研究报道了在大肠杆菌中成功表达并纯化具有活性的重组人源PLCB1蛋白，分析了其酶动力学特性，证实其在G蛋白偶联受体信号传导中的钙离子响应功能。
2. **文献名称**: *"Structural insights into PLCB1 activation by Gαq through recombinant protein crystallography"*
**作者**: Lee C, et al.
**摘要**: 通过重组PLCB1蛋白的结晶和结构解析，揭示了Gαq蛋白激活PLCB1的分子机制，阐明了关键结构域相互作用对酶活性的调控。
3. **文献名称**: *"Development of a mammalian cell-based PLCB1 recombinant system for high-throughput drug screening"*
**作者**: Chen X, et al.
**摘要**: 研究构建了哺乳动物细胞表达的重组PLCB1系统，用于筛选调节其活性的小分子化合物，为针对PLC通路的药物开发提供了高效平台。
(注：以上文献为示例性内容，实际文献需通过学术数据库检索确认。)
**Background of PLCB1 Recombinant Protein**
Phospholipase C beta 1 (PLCB1) is a critical enzyme in intracellular signaling pathways, primarily involved in hydrolyzing phosphatidylinositol 4.5-bisphosphate (PIP2) into secondary messengers inositol 1.4.5-trisphosphate (IP3) and diacylglycerol (DAG). These molecules regulate calcium release from endoplasmic reticulum stores and protein kinase C (PKC) activation, respectively. PLCB1 is a member of the phospholipase C family, which is tightly regulated by G-protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs). Its activity influences diverse cellular processes, including proliferation, differentiation, and apoptosis.
PLCB1 recombinant protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems to ensure proper post-translational modifications and functionality. The recombinant form retains the enzyme’s catalytic domains, enabling researchers to study its structure, kinetics, and interactions with regulatory proteins (e.g., Gαq subunits). It is widely used in *in vitro* assays to investigate GPCR signaling cascades, screen potential modulators, or model diseases linked to PLCB1 dysregulation, such as cancer, bipolar disorder, and neurodegenerative conditions.
The production of PLCB1 recombinant protein often includes affinity tags (e.g., His-tag) for purification and detection. Quality control involves SDS-PAGE, Western blotting, and activity assays using fluorogenic substrates. Its application extends to drug discovery, particularly in targeting PLC pathways for therapeutic intervention. By providing a standardized and scalable source of functional PLCB1. recombinant protein technology enhances mechanistic studies and translational research in cellular signaling.
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