| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLd |
| Uniprot No | P28340 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1107aa |
| 氨基酸序列 | MDGKRRPGPGPGVPPKRARGGLWDDDDAPRPSQFEEDLALMEEMEAEHRL QEQEEEELQSVLEGVADGQVPPSAIDPRWLRPTPPALDPQTEPLIFQQLE IDHYVGPAQPVPGGPPPSHGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYT PAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRESMFG YHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDF EIRFMVDTDIVGCNWLELPAGKYALRLKEKATQCQLEADVLWSDVVSHPP EGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPF LRLALTLRPCAPILGAKVQSYEKEEDLLQAWSTFIRIMDPDVITGYNIQN FDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVS MVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNG NDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRG QQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIAT LDFSSLYPSIMMAHNLCYTTLLRPGTAQKLGLTEDQFIRTPTGDEFVKTS VRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSV YGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAK VVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFP YLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRR LLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAH VELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSL PIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVG GLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEER FSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFG PPGPEAW |
| 预测分子量 | 150 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLδ(DNA聚合酶delta)重组蛋白的3篇代表性文献示例(内容基于学术知识整合，非真实文献)：
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1. **文献名称**：*Structural insights into human DNA polymerase δ in complex with PCNA and DNA*
**作者**：Jain, R. et al.
**摘要**：该研究通过冷冻电镜解析了重组人源POLδ与PCNA蛋白及DNA底物的复合物结构，揭示了POLδ在复制过程中与PCNA的相互作用机制，阐明了其校对功能的结构基础。
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2. **文献名称**：*Reconstitution of human DNA polymerase δ holoenzyme in yeast expression system*
**作者**：Lee, Y.S. & Burgers, P.M.
**摘要**：报道了利用酵母表达系统高效重组表达人源POLδ全酶(含p125、p50、p66亚基)的方法，并验证了其在体外DNA复制和损伤修复中的功能活性，为酶动力学研究提供工具。
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3. **文献名称**：*Functional analysis of POLδ mutations in cancer genomes*
**作者**：Zhao, Y. et al.
**摘要**：通过构建携带肿瘤突变的重组POLδ蛋白，结合生化实验证明特定突变(如POLD1 R689W)导致DNA复制保真度下降，揭示了POLδ缺陷与基因组不稳定性及癌症发生的关系。
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注：以上文献为示例性内容，实际研究需检索PubMed、Web of Science等数据库获取真实文献。
**Background of POLδ Recombinant Protein**
DNA polymerase delta (POLδ) is a crucial eukaryotic enzyme involved in DNA replication and repair, primarily responsible for synthesizing the lagging strand during replication. Comprising four subunits (POLD1. POLD2. POLD3. POLD4), POLδ ensures high-fidelity DNA synthesis due to its proofreading exonuclease activity. Its role extends beyond replication to mismatch repair, base excision repair, and break-induced replication, making it essential for maintaining genomic stability.
Recombinant POLδ proteins are engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to study its structure, function, and interactions. These systems allow large-scale production of individual subunits or complexes, enabling biochemical and structural analyses. For instance, recombinant POLδ has been instrumental in elucidating mechanisms of DNA synthesis fidelity, replication stress responses, and the impact of cancer-associated mutations on enzyme activity.
Recent advancements in cryo-EM and X-ray crystallography have leveraged recombinant POLδ to resolve its 3D structure, revealing conformational changes during DNA binding and catalysis. Additionally, recombinant POLδ variants with specific mutations (e.g., exonuclease-deficient forms) are used to model replication errors and carcinogenesis. In therapeutics, POLδ is explored as a target for anticancer drugs, as inhibitors may selectively target rapidly dividing cancer cells by disrupting replication.
Despite progress, challenges remain, such as reconstituting fully functional multi-subunit complexes and understanding post-translational modifications. Ongoing research aims to refine expression systems and develop assays to study POLδ in chromatin contexts, enhancing its relevance to human health and disease.
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