| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OLFM3 |
| Uniprot No | Q96PB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-478aa |
| 氨基酸序列 | QTLPSLV GLNTTRLSTP DTLTQISPKE GWQVYSSAQD PDGRCICTVV APEQNLCSRD AKSRQLRQLL EKVQNMSQSI EVLNLRTQRD FQYVLKMETQ MKGLKAKFRQ IEDDRKTLMT KHFQELKEKM DELLPLIPVL EQYKTDAKLI TQFKEEIRNL SAVLTGIQEE IGAYDYEELH QRVLSLETRL RDCMKKLTCG KLMKITGPVT VKTSGTRFGA WMTDPLASEK NNRVWYMDSY TNNKIVREYK SIADFVSGAE SRTYNLPFKW AGTNHVVYNG SLYFNKYQSN IIIKYSFDMG RVLAQRSLEY AGFHNVYPYT WGGFSDIDLM ADEIGLWAVY ATNQNAGNIV ISQLNQDTLE VMKSWSTGYP KRSAGESFMI CGTLYVTNSH LTGAKVYYSY STKTSTYEYT DIPFHNQYFH ISMLDYNARD RALYAWNNGH QVLFNVTLFH IIKTEDDT |
| 预测分子量 | 54,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OLFM3重组蛋白的模拟参考文献示例，基于相关领域常见研究方向整理而成：
---
1. **标题**: *"Expression and Purification of Recombinant OLFM3 in Mammalian Cells for Functional Studies"*
**作者**: Zhang Y, Liu X, Chen J.
**摘要**: 本研究成功在HEK293细胞中表达了重组OLFM3蛋白，通过亲和层析纯化并验证其稳定性。实验表明，重组OLFM3可通过调控Wnt/β-catenin通路影响结肠癌细胞增殖。
2. **标题**: *"OLFM3 Recombinant Protein Attenuates Tumor Angiogenesis in a Mouse Xenograft Model"*
**作者**: Wang L, Li H, Kim S.
**摘要**: 利用大肠杆菌表达系统制备重组OLFM3蛋白，发现其能抑制血管内皮细胞迁移和管状结构形成，并通过小鼠模型证实其抑制肿瘤血管生成的作用。
3. **标题**: *"Structural Insights into OLFM3’s Olfactomedin Domain and Its Role in Neurite Outgrowth"*
**作者**: Smith T, Patel R, Lee K.
**摘要**: 通过X射线晶体学解析OLFM3重组蛋白的olfactomedin结构域，揭示其与神经元突触蛋白的相互作用，体外实验证明其促进海马神经元突触生长。
4. **标题**: *"OLFM3 Recombinant Protein Modulates Inflammatory Response in Macrophages via NF-κB Pathway"*
**作者**: Gupta A, Park J, Rodriguez M.
**摘要**: 研究显示，重组OLFM3蛋白可抑制LPS诱导的巨噬细胞炎症因子释放，机制涉及NF-κB信号通路的负向调控，提示其潜在抗炎治疗价值。
---
**说明**：以上文献为模拟示例，实际研究需通过PubMed、Google Scholar等平台以关键词“OLFM3 recombinant protein”检索真实文献。部分研究可能聚焦于OLFM3的疾病关联(如癌症、神经退行性疾病)，重组蛋白多用于机制探索或治疗潜力验证。
OLFM3. or Olfactomedin 3. is a glycoprotein belonging to the olfactomedin domain-containing protein family, which is evolutionarily conserved and implicated in diverse biological processes. Structurally, OLFM3 contains a signal peptide, a coiled-coil domain, and a C-terminal olfactomedin (OLF) domain, a hallmark of this protein family. While its exact physiological role remains under investigation, OLFM3 is suggested to participate in cell adhesion, neural development, and tissue morphogenesis. Dysregulation of OLFM3 has been linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes, highlighting its potential as a therapeutic or diagnostic target.
In cancer biology, OLFM3 is reported to exhibit both tumor-promoting and tumor-suppressive effects depending on the context. For instance, it is overexpressed in colorectal cancer and glioblastoma, where it may enhance cell proliferation and survival via Wnt/β-catenin or Notch signaling pathways. Conversely, reduced OLFM3 expression in prostate cancer correlates with aggressive phenotypes. In neurodegenerative research, OLFM3 is expressed in brain regions like the hippocampus and cortex, potentially influencing synaptic plasticity and neuronal survival. Its interaction with extracellular matrix components suggests roles in neural circuit formation.
Recombinant OLFM3 protein is typically produced using bacterial or mammalian expression systems, enabling functional studies. Purification methods often employ affinity tags (e.g., His-tag) for high yield and purity. Researchers utilize recombinant OLFM3 to investigate its binding partners, signaling mechanisms, and therapeutic potential in vitro and in preclinical models. Despite progress, challenges persist in elucidating its precise molecular interactions and tissue-specific functions, necessitating further structural and mechanistic studies. Overall, OLFM3 represents a multifaceted protein with emerging significance in both basic research and clinical applications.
×
