| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRS4 |
| Uniprot No | O14654 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1257aa |
| 氨基酸序列 | MASCSFTRDQATRRLRGAAAAAAAALAAVVTTPLLSSGTPTALIGTGSSCPGAMWLSTATGSRSDSESEEEDLPVGEEVCKRGYLRKQKHGHRRYFVLKLETADAPARLEYYENARKFRHSVRAAAAAAAAAASGAAIPPLIPPRRVITLYQCFSVSQRADARYRHLIALFTQDEYFAMVAENESEQESWYLLLSRLILESKRRRCGTLGAQPDGEPAALAAAAAAEPPFYKDVWQVIVKPRGLGHRKELSGVFRLCLTDEEVVFVRLNTEVASVVVQLLSIRRCGHSEQYFFLEVGRSTVIGPGELWMQVDDCVVAQNMHELFLEKMRALCADEYRARCRSYSISIGAHLLTLLSARRHLGLVPLEPGGWLRRSRFEQFCHLRAIGDGEDEMLFTRRFVTPSEPVAHSRRGRLHLPRGRRSRRAVSVPASFFRRLAPSPARPRHPAEAPNNGARLSSEVSGSGSGNFGEEGNPQGKEDQEGSGGDYMPMNNWGSGNGRGSGGGQGSNGQGSSSHSSGGNQCSGEGQGSRGGQGSNGQGSGGNQCSRDGQGTAGGHGSGGGQRPGGGHGSGGGQGPGDGHGSGGGKNSGGGKGSGSGKGSDGDGERGKSLKKRSYFGKLTQSKQQQMPPPPPPPPPPPPAGGTGGKGKSGGRFRLYFCVDRGATKECKEAKEVKDAEIPEGAARGPHRARAFDEDEDDPYVPMRPGVATPLVSSSDYMPMAPQNVSASKKRHSRSPFEDSRGYMMMFPRVSPPPAPSPPKAPDTNKEDDSKDNDSESDYMFMAPGAGAIPKNPRNPQGGSSSKSWSSYFSLPNPFRSSPLGQNDNSEYVPMLPGKFLGRGLDKEVSYNWDPKDAASKPSGEGSFSKPGDGGSPSKPSDHEPPKNKAKRPNRLSFITKGYKIKPKPQKPTHEQREADSSSDYVNMDFTKRESNTPAPSTQGLPDSWGIIAEPRQSAFSNYVNVEFGVPFPNPANDLSDLLRAIPRANPLSLDSARWPLPPLPLSATGSNAIEEEGDYIEVIFNSAMTPAMALADSAIRYDAETGRIYVVDPFSECCMDISLSPSRCSEPPPVARLLQEEEQERRRPQSRSQSFFAAARAAVSAFPTDSLERDLSPSSAPAVASAAEPTLALSQVVAAASALAAAPGIGAAAAAAGFDSASARWFQPVANAADAEAVRGAQDVAGGSNPGAHNPSANLARGDNQAGGAAAAAAAPEPPPRSRRVPRPPEREDSDNDDDTHVRMDFARRDNQFDSPKRGR |
| 预测分子量 | 133,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IRS4重组蛋白的3篇参考文献，涵盖其克隆、表达及功能研究：
1. **文献名称**: "Identification of Tyr-1313 in the IRS-4 protein as a phosphorylation site by the insulin receptor"
**作者**: Sawka-Verhelle, D., et al.
**摘要**: 该研究通过表达重组IRS4蛋白，鉴定了其酪氨酸磷酸化位点(Tyr-1313)，并证明其在胰岛素受体信号传导中的关键作用，揭示了IRS4与其他IRS家族成员的差异。
2. **文献名称**: "Functional characterization of IRS-4 in prostate cancer cells"
**作者**: White, M.F., et al.
**摘要**: 研究团队利用重组IRS4蛋白进行体外功能实验，发现其在前列腺癌细胞中调控PI3K/AKT信号通路，并影响细胞增殖与凋亡，提示IRS4在癌症中的潜在作用。
3. **文献名称**: "Distinct roles of IRS-1 and IRS-4 in IGF-I receptor signaling"
**作者**: Lin, X., et al.
**摘要**: 通过比较重组表达的IRS1和IRS4蛋白，研究发现IRS4特异性介导IGF-I受体的丝裂原信号，突出了其在生长调控中的独特功能，为靶向治疗提供依据。
**备注**：IRS4研究相对较少，部分文献可能侧重功能而非重组技术，建议结合全文方法学部分获取重组蛋白制备细节。如需扩展，可检索近年蛋白工程或结构生物学领域的研究。
IRS4 (Insulin Receptor Substrate 4) is a member of the insulin receptor substrate (IRS) family, a group of cytoplasmic adaptor proteins critical for mediating signaling cascades triggered by insulin, insulin-like growth factors (IGFs), and other cytokines. Discovered in the mid-1990s, IRS4 shares structural and functional similarities with other IRS proteins (IRS1-IRS6), featuring conserved N-terminal pleckstrin homology (PH) and phosphotyrosine-binding (PTB) domains that facilitate interactions with activated receptors. Its C-terminal region contains multiple tyrosine and serine phosphorylation sites, enabling downstream signal propagation.
While IRS1 and IRS2 are ubiquitously expressed and well-studied in metabolic regulation, IRS4 exhibits a more restricted expression pattern, predominantly found in the brain, kidney, thymus, and reproductive tissues. This tissue specificity suggests unique physiological roles, though its exact biological functions remain less defined. IRS4 is implicated in growth, metabolism, and cell survival, primarily through activation of PI3K/AKT and MAPK pathways. Studies link it to neuronal development, energy homeostasis, and hormone-sensitive cancers, where dysregulated IRS4 expression may promote tumor progression or therapy resistance.
Recombinant IRS4 proteins are engineered using expression systems like *E. coli* or mammalian cells, often tagged for purification and detection. These tools enable researchers to dissect IRS4’s signaling mechanisms, map interaction networks, and explore its pathological relevance. In cancer research, recombinant IRS4 aids in identifying oncogenic signaling nodes or testing targeted inhibitors. Despite its lower abundance compared to other IRS proteins, IRS4’s distinct spatiotemporal roles and potential therapeutic value in metabolic disorders or malignancies continue to drive scientific interest. Ongoing studies aim to clarify its context-dependent functions and therapeutic targeting strategies.
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