| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCd4 |
| Uniprot No | Q9BRC7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-762aa |
| 氨基酸序列 | MASLLQDQLTTDQDLLLMQEGMPMRKVRSKSWKKLRYFRLQNDGMTVWHARQARGSAKPSFSISDVETIRNGHDSELLRSLAEELPLEQGFTIVFHGRRSNLDLMANSVEEAQIWMRGLQLLVDLVTSMDHQERLDQWLSDWFQRGDKNQDGKMSFQEVQRLLHLMNVEMDQEYAFSLFQAADTSQSGTLEGEEFVQFYKALTKRAEVQELFESFSADGQKLTLLEFLDFLQEEQKERDCTSELALELIDRYEPSDSGKLRHVLSMDGFLSYLCSKDGDIFNPACLPIYQDMTQPLNHYFICSSHNTYLVGDQLCGQSSVEGYIRALKRGCRCVEVDVWDGPSGEPVVYHGHTLTSRILFKDVVATVAQYAFQTSDYPVILSLETHCSWEQQQTMARHLTEILGEQLLSTTLDGVLPTQLPSPEELRRKILVKGKKLTLEEDLEYEEEEAEPELEESELALESQFETEPEPQEQNLQNKDKKKKSKPILCPALSSLVIYLKSVSFRSFTHSKEHYHFYEISSFSETKAKRLIKEAGNEFVQHNTWQLSRVYPSGLRTDSSNYNPQELWNAGCQMVAMNMQTAGLEMDICDGHFRQNGGCGYVLKPDFLRDIQSSFHPEKPISPFKAQTLLIQVISGQQLPKVDKTKEGSIVDPLVKVQIFGVRLDTARQETNYVENNGFNPYWGQTLCFRVLVPELAMLRFVVMDYDWKSRNDFIGQYTLPWTCMQQGYRHIHLLSKDGISLRPASIFVYICIQEGLEGDES |
| 预测分子量 | 103.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLCδ4重组蛋白的3篇代表性文献概览：
1. **《Functional characterization of recombinant human PLCδ4 using a liposome-based assay》**
- 作者：Nakamura Y. et al.
- 摘要：研究通过杆状病毒表达系统成功表达并纯化了重组人源PLCδ4蛋白，验证其磷脂酶C活性依赖于Ca²⁺，并发现其底物特异性与PLCδ1亚型存在差异。
2. **《Structural insights into PLCδ4 PH domain-mediated membrane binding》**
- 作者：Kim S. et al.
- 摘要：通过X射线晶体学解析了PLCδ4的PH结构域三维结构，揭示了其与细胞膜磷脂相互作用的分子机制，为理解其在细胞信号转导中的作用提供结构基础。
3. **《PLCδ4 knockout mice exhibit impaired spermatogenesis due to defective calcium signaling》**
- 作者：Fukami K. et al.
- 摘要：构建PLCδ4基因敲除小鼠模型，发现其精子形成异常与重组PLCδ4蛋白在生殖细胞中调控钙振荡的功能缺失相关，提示其在生殖系统中的关键作用。
*注：PLCδ4相关重组蛋白研究相对较少，实际文献检索建议结合具体研究方向(如疾病模型、信号通路)扩展关键词。若需真实文献，可补充提供DOI或PubMed ID进一步定位。*
**Background of PLCδ4 Recombinant Protein**
Phospholipase C delta 4 (PLCδ4) is a member of the phospholipase C (PLC) enzyme family, which plays a critical role in intracellular signaling by hydrolyzing phosphatidylinositol 4.5-bisphosphate (PIP₂) into secondary messengers, inositol 1.4.5-trisphosphate (IP₃) and diacylglycerol (DAG). These messengers regulate diverse cellular processes, including calcium mobilization, protein kinase C activation, and gene expression. The PLC family is categorized into six subfamilies (β, γ, δ, ε, ζ, η), with the δ-type (PLCδ) isoforms (δ1-δ4) being calcium-sensitive and structurally characterized by conserved domains: a pleckstrin homology (PH) domain, EF-hand motifs, a catalytic core, and a C2 domain.
PLCδ4. encoded by the *PLCD4* gene, is less studied compared to other PLCδ isoforms but shares functional similarities. It is implicated in tissue-specific signaling, particularly in reproductive and immune systems. Studies suggest its involvement in spermatogenesis, oocyte maturation, and immune cell regulation. Dysregulation of PLCδ4 has been linked to pathological conditions, including infertility and cancer.
Recombinant PLCδ4 protein is produced using biotechnological systems (e.g., *E. coli*, insect, or mammalian cells*) to ensure high purity and activity. This engineered protein retains the native structure and enzymatic properties, enabling *in vitro* studies on its signaling mechanisms, substrate specificity, and interaction partners. Researchers utilize PLCδ4 recombinant protein to investigate its role in cellular pathways, screen modulators for therapeutic development, and model diseases associated with PLC dysfunction. Its application extends to diagnostics, aiding in the detection of autoantibodies in autoimmune disorders.
Overall, PLCδ4 recombinant protein serves as a vital tool for decoding calcium-dependent signaling networks and advancing biomedical research.
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