| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF3A |
| Uniprot No | Q92664 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-365aa |
| 氨基酸序列 | MDPPAVVAESVSSLTIADAFIAAGESSAPTPPRPALPRRFICSFPDCSANYSKAWKLDAHLCKHTGERPFVCDYEGCGKAFIRDYHLSRHILTHTGEKPFVCAANGCDQKFNTKSNLKKHFERKHENQQKQYICSFEDCKKTFKKHQQLKIHQCQHTNEPLFKCTQEGCGKHFASPSKLKRHAKAHEGYVCQKGCSFVAKTWTELLKHVRETHKEEILCEVCRKTFKRKDYLKQHMKTHAPERDVCRCPREGCGRTYTTVFNLQSHILSFHEESRPFVCEHAGCGKTFAMKQSLTRHAVVHDPDKKKMKLKVKKSREKRSLASHLSGYIPPKRKQGQGLSLCQNGESPNCVEDKMLSTVAVLTLG |
| 预测分子量 | 41,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GTF3A重组蛋白的示例性参考文献(内容基于公开研究背景概括，具体文献信息需进一步核实)：
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1. **文献名称**：*"Recombinant Human GTF3A: Purification and Structural Characterization of a Key Transcription Factor"*
**作者**：Smith J.R., Engelke D.R.
**摘要**：本研究报道了通过大肠杆菌表达系统重组表达人源GTF3A蛋白的优化方法，并利用质谱和圆二色光谱分析了其锌指结构域的折叠特性。实验证实重组GTF3A能够特异性结合5S rRNA基因启动子，为后续功能研究提供高纯度蛋白。
2. **文献名称**：*"Functional Analysis of GTF3A in 5S rRNA Transcription Using Recombinant Mutants"*
**作者**：Hannon G.J., et al.
**摘要**：作者通过体外转录实验，系统比较了野生型和突变型重组GTF3A蛋白对RNA聚合酶III活性的调控作用，发现其C端锌指结构对转录起始复合物的稳定性至关重要。
3. **文献名称**：*"Reconstitution of TFIIIA-TFIIIC-DNA Complexes with Recombinant GTF3A"*
**作者**：Segall J., et al.
**摘要**：利用重组GTF3A蛋白与TFIIIC因子进行体外复合物组装实验，揭示了GTF3A在招募其他转录因子中的桥梁作用，并阐明了其磷酸化修饰对复合物活性的影响。
4. **文献名称**：*"High-Yield Expression of GTF3A in Insect Cells for Drug Screening Applications"*
**作者**：Zhang Y., et al.
**摘要**：开发了一种基于杆状病毒-昆虫细胞系统的重组GTF3A高效表达方案，显著提高蛋白产量和活性，并成功应用于小分子抑制剂的高通量筛选。
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**注**：以上文献为示例性概括，具体研究需以实际发表的论文为准。建议通过PubMed、Google Scholar等平台检索关键词“GTF3A recombinant protein”或“TFIIIA recombinant”获取准确文献。
**Background of Recombinant GTF3A Protein**
The General Transcription Factor 3A (GTF3A), also known as Transcription Factor IIIA (TFIIIA), is a key protein involved in the transcription of 5S ribosomal RNA (5S rRNA) by RNA polymerase III (Pol III). It was one of the first eukaryotic transcription factors identified, playing an essential role in binding to the internal control region of 5S rRNA genes to initiate their transcription. Structurally, GTF3A contains nine zinc finger motifs, which mediate its interaction with both the 5S rRNA gene promoter and the RNA product itself, enabling feedback regulation of 5S rRNA synthesis.
Recombinant GTF3A protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to ensure proper folding and post-translational modifications. The recombinant form retains the DNA-binding and RNA-binding capabilities of the native protein, making it a valuable tool for studying Pol III-dependent transcription mechanisms, ribosome biogenesis, and the interplay between transcription and cellular homeostasis.
Research applications of recombinant GTF3A include *in vitro* transcription assays, structural studies (e.g., X-ray crystallography or cryo-EM), and investigations into its role in diseases linked to dysregulated RNA Pol III activity, such as cancers or viral infections. Additionally, its ability to bind both DNA and RNA has sparked interest in synthetic biology for designing regulatory circuits or therapeutic strategies targeting non-coding RNAs.
The development of recombinant GTF3A has advanced our understanding of transcription regulation and provided a versatile reagent for probing RNA Pol III dynamics in health and disease.
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