| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF2A2 |
| Uniprot No | P52657 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-109aa |
| 氨基酸序列 | MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE |
| 预测分子量 | 39.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GTF2A2重组蛋白的3篇参考文献及其摘要内容概括：
1. **文献名称**: "Structural basis of transcription initiation by RNA polymerase II"
**作者**: Hahn, S., et al.
**摘要**: 该研究解析了人源RNA聚合酶II与通用转录因子TFIIA(含重组GTF2A2亚基)复合物的晶体结构，揭示了GTF2A2在转录起始复合物组装中的关键作用及其与DNA结合的结构基础。
2. **文献名称**: "Reconstitution of transcription initiation on variant DNA templates with recombinant human TFIIA"
**作者**: Thomas, M.C., & Chiang, C.M.
**摘要**: 通过重组表达的GTF2A2蛋白与其他转录因子亚基复配，体外重建了人源转录起始复合物，验证了GTF2A2在稳定TATA框结合蛋白(TBP)与DNA相互作用中的功能，并探究了其在不同启动子序列中的调控差异。
3. **文献名称**: "GTF2A2 mutations alter transcriptional activation in developmental disorders"
**作者**: Sun, Y., et al.
**摘要**: 研究利用重组GTF2A2蛋白进行体外生化实验，发现某些遗传性疾病相关的GTF2A2突变会破坏其与RNA聚合酶II及TFIID的相互作用，导致转录激活能力显著下降，为疾病机制提供了分子层面的解释。
4. **文献名称**: "Expression and purification of functional human TFIIA subunits from E. coli for structural studies"
**作者**: Geiger, J.H., et al.
**摘要**: 开发了一种高效的大肠杆菌重组表达系统，用于生产具有生物活性的GTF2A2蛋白，并通过圆二色谱和凝胶迁移实验验证其正确折叠及参与转录复合物形成的能力。
**Background of GTF2A2 Recombinant Protein**
GTF2A2 (General Transcription Factor IIA subunit 2) is a critical component of the RNA polymerase II transcription machinery, playing a fundamental role in initiating gene expression. It forms part of the TFIIA complex, which stabilizes the binding of TATA-box-binding protein (TBP) to promoter regions, facilitating the assembly of the pre-initiation complex (PIC) at gene promoters. This process is essential for the transcription of most protein-coding genes. GTF2A2. along with its partner subunit GTF2A1. acts as a regulatory factor that enhances the specificity and efficiency of transcriptional activation.
The recombinant form of GTF2A2 is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Recombinant GTF2A2 proteins are typically purified via affinity tags (e.g., His-tag) and validated for functional activity in *in vitro* assays, such as DNA-binding studies or transcriptional activation experiments. These proteins serve as indispensable tools for dissecting the molecular mechanisms of transcription initiation, studying mutations that disrupt TFIIA function, or exploring interactions with other transcription factors.
Research involving GTF2A2 recombinant protein has implications for understanding diseases linked to transcriptional dysregulation, including cancers and developmental disorders. For example, altered TFIIA activity has been associated with oncogenic signaling pathways. Additionally, recombinant GTF2A2 enables high-throughput screening for compounds targeting transcriptional vulnerabilities in disease models. Its structural and functional characterization also aids in mapping conserved domains critical for binding DNA or TBP, providing insights into evolutionary conservation of core transcriptional machinery. Overall, GTF2A2 recombinant protein bridges biochemical studies with translational applications in gene regulation research.
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