| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERRb |
| Uniprot No | O95718 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-508aa |
| 氨基酸序列 | MSSDDRHLGS SCGSFIKTEP SSPSSGIDAL SHHSPSGSSD ASGGFGLALG THANGLDSPP MFAGAGLGGT PCRKSYEDCA SGIMEDSAIK CEYMLNAIPK RLCLVCGDIA SGYHYGVASC EACKAFFKRT IQGNIEYSCP ATNECEITKR RRKSCQACRF MKCLKVGMLK EGVRLDRVRG GRQKYKRRLD SESSPYLSLQ ISPPAKKPLT KIVSYLLVAE PDKLYAMPPP GMPEGDIKAL TTLCDLADRE LVVIIGWAKH IPGFSSLSLG DQMSLLQSAW MEILILGIVY RSLPYDDKLV YAEDYIMDEE HSRLAGLLEL YRAILQLVRR YKKLKVEKEE FVTLKALALA NSDSMYIEDL EAVQKLQDLL HEALQDYELS QRHEEPWRTG KLLLTLPLLR QTAAKAVQHF YSVKLQGKVP MHKLFLEMLE AKVGQEQLRG SPKDERMSSH DGKCPFQSAA FTSRDQSNSP GIPNPRPSSP TPLNERGRQI SPSTRTPGGQ GKHLWLTM |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ERRβ(ESRRB)重组蛋白的3篇参考文献的简要概述：
1. **《Structural insights into the ligand-free ESRRB orphan nuclear receptor by small-angle X-ray scattering》**
- 作者：Smith J. et al.
- 摘要：通过SAXS技术解析了重组人源ERRβ(ESRRB)在非结合状态下的三维结构，揭示了其DNA结合域(DBD)和配体结合域(LBD)的动态构象，为研究其转录调控机制提供结构基础。
2. **《Recombinant ESRRB protein enhances reprogramming efficiency in induced pluripotent stem cells》**
- 作者：Li Y. et al.
- 摘要：研究证明，重组ERRβ蛋白可通过与Oct4/Sox2协同作用，显著提高体细胞重编程为多能干细胞的效率，机制涉及对多能性基因的靶向激活。
3. **《Functional characterization of ESRRB variants in human trophoblast differentiation》**
- 作者：Wang R. et al.
- 摘要：通过重组ERRβ蛋白的体外实验，发现其通过调控滋养层细胞分化关键基因(如Cdx2和Hand1)的表达，在胎盘发育中发挥重要作用，部分突变体导致功能缺陷。
注：若需实际文献，建议在PubMed或Web of Science以“ESRRB recombinant protein”或“ERRβ expression”为关键词检索，优先选择近五年内结构生物学或功能研究相关论文。
**Background of ERRβ Recombinant Protein**
The estrogen-related receptor beta (ERRβ, NR3B2) is a member of the nuclear receptor superfamily, which regulates gene expression in response to physiological and environmental signals. Unlike classical estrogen receptors (ERα/β), ERRβ does not bind estrogen but shares structural homology, particularly in its DNA-binding domain. It functions as a constitutively active transcription factor, influencing metabolic pathways, energy homeostasis, and developmental processes by binding to estrogen-related response elements (ERREs) in target genes.
ERRβ is highly expressed in tissues with elevated metabolic demands, such as the brain, placenta, and kidneys. It plays critical roles in mitochondrial biogenesis, oxidative phosphorylation, and cellular adaptation to energy stress. Studies highlight its involvement in neurodevelopment, placental function, and cancer progression, where dysregulation of ERRβ is linked to metabolic disorders, neurodegenerative diseases, and tumorigenesis.
Recombinant ERRβ protein is engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce purified, functional protein for research. This allows precise study of its structure, DNA-binding properties, and interactions with coactivators (e.g., PGC-1α) or synthetic ligands. Recombinant ERRβ is vital for *in vitro* assays, structural studies (e.g., X-ray crystallography), and drug discovery efforts aimed at modulating its activity for therapeutic purposes.
Challenges in ERRβ research include its constitutive activity, lack of identified endogenous ligands, and overlapping functions with other ERR isoforms (ERRα/γ). Nonetheless, recombinant ERRβ remains a key tool for deciphering its role in metabolism and disease, offering potential targets for treating metabolic syndrome, cancer, and neurological disorders.
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