| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNTNAP5 |
| Uniprot No | Q8WYK1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1306aa |
| 氨基酸序列 | MDSLPRLTSVLTLLFSGLWHLGLTATNYNCDDPLASLLSPMAFSSSSDLTGTHSPAQLNWRVGTGGWSPADSNAQQWLQMDLGNRVEITAVATQGRYGSSDWVTSYSLMFSDTGRNWKQYKQEDSIWTFAGNMNADSVVHHKLLHSVRARFVRFVPLEWNPSGKIGMRVEVYGCSYKSDVADFDGRSSLLYRFNQKLMSTLKDVISLKFKSMQGDGVLFHGEGQRGDHITLELQKGRLALHLNLGDSKARLSSSLPSATLGSLLDDQHWHSVLIERVGKQVNFTVDKHTQHFRTKGETDALDIDYELSFGGIPVPGKPGTFLKKNFHGCIENLYYNGVNIIDLAKRRKHQIYTGNVTFSCSEPQIVPITFVNSSGSYLLLPGTPQIDGLSVSFQFRTWNKDGLLLSTELSEGSGTLLLSLEGGILRLVIQKMTERVAEILTGSNLNDGLWHSVSINARRNRITLTLDDEAAPPAPDSTWVQIYSGNSYYFGGCPDNLTDSQCLNPIKAFQGCMRLIFIDNQPKDLISVQQGSLGNFSDLHIDLCSIKDRCLPNYCEHGGSCSQSWTTFYCNCSDTSYTGATCHNSIYEQSCEVYRHQGNTAGFFYIDSDGSGPLGPLQVYCNITEDKIWTSVQHNNTELTRVRGANPEKPYAMALDYGGSMEQLEAVIDGSEHCEQEVAYHCRRSRLLNTPDGTPFTWWIGRSNERHPYWGGSPPGVQQCECGLDESCLDIQHFCNCDADKDEWTNDTGFLSFKDHLPVTQIVITDTDRSNSEAAWRIGPLRCYGDRRFWNAVSFYTEASYLHFPTFHAEFSADISFFFKTTALSGVFLENLGIKDFIRLEISSPSEITFAIDVGNGPVELVVQSPSLLNDNQWHYVRAERNLKETSLQVDNLPRSTRETSEEGHFRLQLNSQLFVGGTSSRQKGFLGCIRSLHLNGQKMDLEERAKVTSGVRPGCPGHCSSYGSICHNGGKCVEKHNGYLCDCTNSPYEGPFCKKEVSAVFEAGTSVTYMFQEPYPVTKNISLSSSAIYTDSAPSKENIALSFVTTQAPSLLLFINSSSQDFVVVLLCKNGSLQVRYHLNKEETHVFTIDADNFANRRMHHLKINREGRELTIQMDQQLRLSYNFSPEVEFRVIRSLTLGKVTENLGLDSEVAKANAMGFAGCMSSVQYNHIAPLKAALRHATVAPVTVHGTLTESSCGFMVDSDVNAVTTVHSSSDPFGKTDEREPLTNAVRSDSAVIGGVIAVVIFIIFCIIGIMTRFLYQHKQSHRTSQMKEKEYPENLDSSFRNEIDLQNTVSECKREYFI |
| 预测分子量 | 145,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNTNAP5重组蛋白的参考文献示例(注：以下内容为假设性概括，实际文献需通过学术数据库查询确认)：
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1. **标题**: *"Expression and Functional Characterization of Recombinant CNTNAP5 in Mammalian Cells"*
**作者**: Smith A, et al.
**摘要**: 研究报道了CNTNAP5重组蛋白在HEK293细胞中的表达与纯化，并发现该蛋白通过其细胞外结构域介导神经元间的黏附作用，可能参与突触形成调控。
2. **标题**: *"Structural Insights into CNTNAP5’s Role in Neural Development via Recombinant Protein Analysis"*
**作者**: Jones B, et al.
**摘要**: 利用重组CNTNAP5蛋白进行X射线晶体学分析，揭示了其胞内PDZ结合域的关键结构，并证明其与突触后蛋白的相互作用影响神经环路发育。
3. **标题**: *"CNTNAP5 Recombinant Protein Mutants Link to Autism Spectrum Disorder Pathogenesis"*
**作者**: Lee C, et al.
**摘要**: 通过构建CNTNAP5重组蛋白的罕见错义突变体，发现特定突变导致其与配体结合能力下降，提示其在自闭症相关信号通路中的潜在作用。
4. **标题**: *"Optimization of CNTNAP5 Recombinant Production in Baculovirus System for High-Throughput Screening"*
**作者**: Brown D, et al.
**摘要**: 描述在昆虫杆状病毒系统中高效表达CNTNAP5重组蛋白的工艺优化，成功应用于药物筛选平台，以靶向神经精神疾病相关通路。
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建议通过PubMed或Google Scholar以关键词 **"CNTNAP5 recombinant protein"** 或 **"CNTNAP5 expression function"** 检索最新文献获取准确信息。
**Background of CNTNAP5 Recombinant Protein**
CNTNAP5 (Contactin-associated protein-like 5) is a member of the neurexin superfamily, a group of transmembrane proteins predominantly involved in cell adhesion and synaptic organization within the nervous system. Structurally, CNTNAP5 contains conserved domains such as laminin G-like repeats, epidermal growth factor (EGF)-like motifs, and a discoidin domain, which mediate protein-protein interactions critical for neural development and connectivity. While its paralogs, like CNTNAP2. are well-studied in neurodevelopmental disorders (e.g., autism spectrum disorder), CNTNAP5 remains less characterized, though it is hypothesized to play roles in neuronal signaling, synapse formation, or glial-neuronal communication.
Recombinant CNTNAP5 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional domains or full-length proteins for experimental studies. This tool enables researchers to investigate CNTNAP5’s biochemical properties, ligand-binding capabilities, and interactions with partners like contactins or extracellular matrix components. Its applications span structural biology (e.g., crystallography), *in vitro* binding assays, and antibody development. Additionally, recombinant CNTNAP5 aids in exploring its potential involvement in neurological diseases or as a biomarker.
Despite limited functional data, emerging genomic studies link CNTNAP5 variations to neuropsychiatric conditions, underscoring its biological relevance. Further research using recombinant protein models could clarify its mechanistic contributions to neural circuits and disease pathways, bridging gaps in understanding neurexin-related networks.
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