| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDK18 |
| Uniprot No | Q07002 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-472aa |
| 氨基酸序列 | MNKMKNFKRRFSLSVPRTETIEESLAEFTEQFNQLHNRRNENLQLGPLGR DPPQECSTFSPTDSGEEPGQLSPGVQFQRRQNQRRFSMEDVSKRLSLPMD IRLPQEFLQKLQMESPDLPKPLSRMSRRASLSDIGFGKLETYVKLDKLGE GTYATVFKGRSKLTENLVALKEIRLEHEEGAPCTAIREVSLLKNLKHANI VTLHDLIHTDRSLTLVFEYLDSDLKQYLDHCGNLMSMHNVKIFMFQLLRG LAYCHHRKILHRDLKPQNLLINERGELKLADFGLARAKSVPTKTYSNEVV TLWYRPPDVLLGSTEYSTPIDMWGVGCIHYEMATGRPLFPGSTVKEELHL IFRLLGTPTEETWPGVTAFSEFRTYSFPCYLPQPLINHAPRLDTDGIHLL SSLLLYESKSRMSAEAALSHSYFRSLGERVHQLEDTASIFSLKEIQLQKD PGYRGLAFQQPGRGKNRRQSIF |
| 预测分子量 | 76 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDK18重组蛋白的模拟参考文献(部分信息为示例性概括，实际文献需通过学术数据库核实)：
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1. **文献名称**：*CDK18 regulates DNA replication and repair through interaction with BRCA1*
**作者**：Zhang Y, et al.
**摘要**：本研究利用重组CDK18蛋白进行体外激酶实验，发现CDK18通过磷酸化BRCA1的特定结构域，增强其在DNA损伤修复中的功能。重组蛋白在大肠杆菌中表达并纯化，实验证实其激酶活性依赖于细胞周期阶段。
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2. **文献名称**：*Structural and functional characterization of recombinant CDK18-cyclin complexes*
**作者**：Lee S, Kim JH.
**摘要**：作者通过昆虫杆状病毒系统表达并纯化CDK18与不同cyclin的复合体，解析了其晶体结构。功能研究表明，CDK18-cyclin B复合体对RNA聚合酶II的C端结构域具有磷酸化活性，提示其在转录调控中的作用。
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3. **文献名称**：*Development of a high-throughput assay for CDK18 inhibitors using recombinant protein*
**作者**：Thompson R, et al.
**摘要**：文章报道了一种基于重组CDK18蛋白的高通量激酶活性检测平台，用于筛选小分子抑制剂。实验验证了多个候选化合物对CDK18的抑制作用，并评估其抗癌潜力。
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4. **文献名称**：*CDK18 deficiency alters cell cycle progression and chromatin dynamics*
**作者**：Gómez V, et al.
**摘要**：通过对比野生型和CDK18敲除细胞，研究发现CDK18重组蛋白可挽救细胞周期G2/M期阻滞表型，表明其在染色质重塑和细胞周期检查点中的关键作用。
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**备注**：以上文献信息为示例性内容，实际研究中请通过**PubMed**或**Web of Science**等数据库，以“CDK18 recombinant protein”或“CDK18 kinase activity”为关键词检索最新文献。CDK18的研究相对较少，建议结合其同家族蛋白(如CDK12/13)的相关机制扩展阅读。
**Background of CDK18 Recombinant Protein**
CDK18 (Cyclin-Dependent Kinase 18) is a serine/threonine kinase belonging to the CDK family, which regulates critical cellular processes such as cell cycle progression, transcription, and DNA repair. Unlike canonical CDKs (e.g., CDK1. CDK2), CDK18 is classified as a transcriptional CDK due to its role in modulating RNA polymerase II activity and mRNA processing. It interacts with cyclin partners (e.g., cyclin E or cyclin K) to phosphorylate target substrates, influencing gene expression and cellular responses to stress.
CDK18 has garnered attention for its potential involvement in cancer and other diseases. Studies suggest its overexpression or dysregulation may contribute to tumorigenesis, particularly in breast cancer, ovarian cancer, and glioblastoma, where it may promote cell proliferation or chemoresistance. Conversely, CDK18 also exhibits context-dependent tumor-suppressive roles, highlighting its functional complexity.
Recombinant CDK18 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and activity. It typically includes tags (e.g., His-tag) for affinity purification and detection. This recombinant tool enables researchers to study CDK18's enzymatic activity, substrate specificity, and interactions with inhibitors or therapeutic compounds.
Applications span drug discovery, biochemical assays (e.g., kinase activity profiling), and structural studies to design selective CDK18 inhibitors. Additionally, it aids in elucidating CDK18's role in signaling pathways (e.g., RB/E2F or PI3K/AKT) and its crosstalk with other CDKs. As aberrant CDK activity is a hallmark of many cancers, CDK18 recombinant protein serves as a vital resource for exploring targeted therapies and biomarker development.
In summary, CDK18 recombinant protein bridges basic research and translational medicine, offering insights into CDK18's dual roles in health and disease while supporting therapeutic innovation.
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