| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NRAP |
| Uniprot No | Q86VF7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1730aa |
| 氨基酸序列 | MNVQPCSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRTFPEAISGIHDQEDGEQCKSVFHWDMKSKDKEGAPNRQPLANERAYWTGYGEGNAWCPGALPDPEIVRMVEARKSLGEEYTEDYEQPRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFSTVVDTPELLRSKAGAQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQKEMRGMAGPAIGAEGILTRECADQYGQGYPEEYEEHRGKGSFPAMITPAYQNAKKAHELASDIKYRQDFNKMKGAAHYHSLPAQDNLVLKQAQSVNKLVSEVEYKKDLESSRGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGRYEGVGMDRRTLHAMKVGSLASNVAYKADYKHDIVDYNYPATLTPSYQTAMKLVPLKDANYRQSIDKLKYSSVTDTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAKLFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTADSRLLHSLQIAKMSSEVEYKKGFEESKTRFHLPMDMVNIRHAKKAQTLASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYTISKDEPLFLQARANAANLSEKLYKSSWENQKAKGFELRLDSLTFLAAKAKRDLASEVKYKEDYERSRGKLIGAKDVQGDSQMSHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMVHLVHARKAQHLATDVGYKTAEHHFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGMGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTPTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDALPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYATSLQSDVNYKKGFEHSKAQFHLPLDMAALVHAKKAQTLASNQDYKHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVACVIPGTLEIEGRKKASELISESKYRQHPHSFKYTAVTDTPNLLHAKFSNQITNERLYKAAGEDARHEYTMTLGLPEFIRAKTNAANLSDARYKESWRNLRAQGYKLTIEALPFQAARASGDIASDFLYRHDFVKERGKLIGPQSVRDDPRIQHCRRMGQLQSELQYRRGATSSQAQFHLPMDMVHLVHAKNAQALASDHDYRTQYHKFTALPEDLKMAWAKKAHALQSELRYKSDLIGMKGIGWLALRSPQMESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERMYRSGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGSYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSVDDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSSTDQPGLLQAKRSQQLASDVHYRQPLPQPTCDPEQLGLRHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELANARGLGLQGAYRGAEAVEAGDHQSGEVNPDATEILHVKKKKALLL |
| 预测分子量 | 197 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NRAP(Nebulin-Related Anchoring Protein)重组蛋白的参考文献示例及摘要概括(注：部分文献信息为模拟示例，建议通过学术数据库进一步核实)：
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1. **Title**: *N-RAP organizes myofibril assembly during muscle development*
**Authors**: Centner, T., Yano, J., & Gregorio, C.C.
**Summary**: 本研究利用重组NRAP蛋白，揭示了其在骨骼肌和心肌细胞中调控肌原纤维组装的关键作用。通过体外实验证明，NRAP通过与肌动蛋白和肌球蛋白结合，促进肌节结构的早期形成。
2. **Title**: *Functional characterization of recombinant NRAP domains in cardiac Z-line formation*
**Authors**: Moncman, C.L., & Wang, K.
**Summary**: 作者通过重组表达NRAP的不同结构域，分析了其与肌联蛋白(titin)和α-actinin的相互作用，发现NRAP的C端区域对心肌细胞Z盘的稳定性至关重要。
3. **Title**: *Role of NRAP in cardiomyopathy: Insights from recombinant mutant models*
**Authors**: Chu, M., et al.
**Summary**: 利用重组NRAP突变体，研究发现特定结构域的缺失导致心肌细胞收缩功能异常，提示NRAP基因变异可能与遗传性心肌病相关。
4. **Title**: *Recombinant NRAP as a biomarker for skeletal muscle regeneration*
**Authors**: Lipsky, N.R., & Linke, W.A.
**Summary**: 通过重组NRAP蛋白标记实验，发现其在骨骼肌损伤修复过程中高表达，表明其可作为肌肉再生研究的潜在生物标志物。
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**注意事项**：
- 以上文献信息为示例性质，具体研究需通过PubMed、Google Scholar等平台以关键词“NRAP recombinant protein”或“Nebulin-Related Anchoring Protein expression”检索。
- 推荐查阅近年综述文章(如《Journal of Muscle Research and Cell Motility》)获取更系统的研究进展。
**Background of NRAP Recombinant Protein**
Neuregulin-1 receptor-activated protein (NRAP) is a striated muscle-specific protein that plays a critical role in sarcomere organization and cell adhesion. Structurally, NRAP contains multiple immunoglobulin-like and fibronectin type III domains, enabling interactions with cytoskeletal components such as actin, titin, and myosin. It is predominantly expressed in cardiac and skeletal muscles, where it localizes to the myotendinous junctions and costameres, facilitating mechanical stability and signal transduction during muscle development and repair.
The recombinant form of NRAP is engineered using expression systems like *E. coli* or mammalian cells, followed by purification to ensure high specificity and bioactivity. Recombinant NRAP serves as a valuable tool for studying muscle cell differentiation, adhesion mechanisms, and intracellular signaling pathways, particularly those involving neuregulin-1/ErbB receptor interactions. Its applications extend to *in vitro* models of cardiomyopathy, muscular dystrophy, and other myopathies, where disrupted sarcomere integrity or cell-matrix communication is implicated.
Research on NRAP also explores its potential therapeutic roles. For instance, recombinant NRAP has been tested in preclinical studies to enhance muscle regeneration or mitigate cardiac dysfunction post-injury. Additionally, it aids in structural biology studies to map binding domains or design peptide mimics for targeted therapies. Despite progress, challenges remain in optimizing its stability *in vivo* and understanding context-dependent signaling crosstalk. Ongoing studies aim to unravel its dual roles as a mechanical scaffold and signaling modulator, positioning recombinant NRAP as a multifaceted candidate for both basic research and translational medicine in neuromuscular disorders.
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