| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTRR |
| Uniprot No | Q9UBK8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-725aa |
| 氨基酸序列 | MGAASVRAGARLVEVALCSFTVTCLEVMRRFLLLYATQQGQAKAIAEEICEQAVVHGFSADLHCISESDKYDLKTETAPLVVVVSTTGTGDPPDTARKFVKEIQNQTLPVDFFAHLRYGLLGLGDSEYTYFCNGGKIIDKRLQELGARHFYDTGHADDCVGLELVVEPWIAGLWPALRKHFRSSRGQEEISGALPVASPASSRTDLVKSELLHIESQVELLRFDDSGRKDSEVLKQNAVNSNQSNVVIEDFESSLTRSVPPLSQASLNIPGLPPEYLQVHLQESLGQEESQVSVTSADPVFQVPISKAVQLTTNDAIKTTLLVELDISNTDFSYQPGDAFSVICPNSDSEVQSLLQRLQLEDKREHCVLLKIKADTKKKGATLPQHIPAGCSLQFIFTWCLEIRAIPKKAFLRALVDYTSDSAEKRRLQELCSKQGAADYSRFVRDACACLLDLLLAFPSCQPPLSLLLEHLPKLQPRPYSCASSSLFHPGKLHFVFNIVEFLSTATTEVLRKGVCTGWLALLVASVLQPNIHASHEDSGKALAPKISISPRTTNSFHLPDDPSIPIIMVGPGTGIAPFIGFLQHREKLQEQHPDGNFGAMWLFFGCRHKDRDYLFRKELRHFLKHGILTHLKVSFSRDAPVGEEEAPAKYVQDNIQLHGQQVARILLQENGHIYVCGDAKNMAKDVHDALVQIISKEVGVEKLEAMKTLATLKEEKRYLQDIWS |
| 预测分子量 | 82.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Human methionine synthase reductase, a pivotal enzyme in folate and vitamin B12 metabolism: Cloning and characterization"** by Leclerc et al.
摘要：该研究首次克隆并表征了人类MTRR基因，阐明其在叶酸和维生素B12代谢中的核心作用，尤其是修复甲硫氨酸合成酶(MTR)活性所需的还原功能。
2. **"The interaction of methionine synthase reductase with methionine synthase: Structural insights into reactivation and vitamin B12 trafficking"** by Gherasim et al.
摘要：通过结构生物学分析，揭示了MTRR与MTR蛋白的相互作用机制，解释了其如何通过还原作用重新激活MTR，并参与维生素B12的转运与循环。
3. **"Pathogenic mutations affecting the methionine synthase reductase (MTRR) gene: Functional analysis and implications for homocysteine metabolism"** by Olteanu & Banerjee
摘要：研究鉴定了MTRR基因的致病突变，证明其会导致酶活性丧失，引发高同型半胱氨酸血症，并探讨了其与心血管疾病及神经发育障碍的关联。
4. **"MTRR deficiency disrupts mitochondrial function and lipid metabolism via aberrant methylation in embryonic development"** by Zhu et al.
摘要：利用动物模型发现MTRR缺陷通过干扰甲基化过程，导致线粒体功能障碍和脂代谢异常，揭示了其在胚胎发育及先天畸形(如神经管缺陷)中的关键作用。
**Background of MTRR (Methionine Synthase Reductase) Recombinant Protein**
Methionine synthase reductase (MTRR) is a critical enzyme in the folate and methionine cycles, essential for maintaining cellular methylation processes and nucleotide synthesis. It functions as a partner to methionine synthase (MTR), regenerating the active form of vitamin B12 (cobalamin) required for MTR to catalyze the remethylation of homocysteine to methionine. This reaction is vital for producing S-adenosylmethionine (SAM), the primary methyl donor for DNA, protein, and lipid methylation, which regulates gene expression, DNA repair, and cellular signaling.
MTRR is a flavoprotein belonging to the ferredoxin-NADP⁺ reductase (FNR) family, utilizing NADPH as an electron donor to reduce oxidized cob(II)alamin in MTR. Mutations in the *MTRR* gene disrupt this process, leading to hyperhomocysteinemia, impaired methylation, and folate cycle dysfunction. Such defects are linked to developmental disorders (e.g., neural tube defects), cardiovascular diseases, and neurodegenerative conditions.
Recombinant MTRR protein, produced via expression systems like *E. coli* or mammalian cells, enables detailed study of its structure-function relationships, enzymatic kinetics, and interaction with MTR. It serves as a tool to investigate genetic variants, assess therapeutic strategies for methylation-related disorders, and develop diagnostic assays. Research on recombinant MTRR also explores its potential in mitigating oxidative stress, as disrupted methylation cycles elevate homocysteine, a risk factor for cellular damage. Overall, MTRR recombinant protein is pivotal for advancing understanding of folate metabolism and associated pathologies.
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