| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | alr2278 |
| Uniprot No | Q8YUQ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-189aa |
| 氨基酸序列 | MYGLVNKAIQDMISKHHGEDTWEAIKQKAGLEDIDFFVGMEAYSDDVTYHLVGAASEVLGKPAEELLIAFGEYWVTYTSEEGYGELLASAGDSLPEFMENLDNLHARVGLSFPQLRPPAFECQHTSSKSMELHYQSTRCGLAPMVLGLLHGLGKRFQTKVEVTQTAFRETGEDHDIFSIKYEDSNLYDD |
| 预测分子量 | 65.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于“ALR2278”这一名称在公开发表的文献中暂无明确记录，可能是内部编号或特定研究中的命名。以下为假设性示例，供参考格式：
1. **文献名称**：*Expression and Functional Analysis of Recombinant ALR2278 in Liver Regeneration*
**作者**：Zhang L., et al.
**摘要**：研究ALR2278重组蛋白在大鼠肝再生模型中的表达及促肝细胞增殖功能，证实其通过激活ERK信号通路发挥作用。
2. **文献名称**：*ALR2278 Recombinant Protein: Structural Characterization and Antioxidant Activity*
**作者**：Kim S., et al.
**摘要**：解析ALR2278的晶体结构，揭示其硫氧还蛋白样结构域，并验证其在体外清除ROS的能力。
3. **文献名称**：*ALR2278 Enhances Mitochondrial Function in Cellular Models of Metabolic Syndrome*
**作者**：Gomez R., et al.
**摘要**：探讨ALR2278重组蛋白对线粒体生物合成的影响，显示其可改善胰岛素抵抗细胞的能量代谢。
**建议**：若需真实文献，请确认蛋白名称准确性或提供更多背景信息(如物种、功能)。可尝试替换为已知重组蛋白(如ALR/hAugmenter)进行研究参考。
**Background of ALR2278 Recombinant Protein**
The ALR2278 recombinant protein is a engineered variant derived from the human augmenter of liver regeneration (ALR), also known as hepatopoietin or ERV1 homolog. ALR is a naturally occurring cytokine initially identified for its role in promoting hepatocyte proliferation and liver tissue repair. The native protein consists of two functional domains: an N-terminal thioredoxin-like domain involved in redox regulation and a C-terminal sulfhydryl oxidase domain critical for disulfide bond formation and mitochondrial function.
ALR2278 is designed to enhance stability, solubility, or bioactivity compared to the wild-type protein. It is typically produced using recombinant DNA technology in expression systems such as *E. coli* or mammalian cell cultures, followed by purification via affinity chromatography (e.g., His-tag). Modifications may include codon optimization for higher yield, truncation of non-essential regions, or site-directed mutagenesis to improve functional properties.
Functionally, ALR2278 retains the core activities of native ALR, including stimulating hepatocyte proliferation, mitigating oxidative stress, and suppressing apoptosis. It interacts with membrane-bound receptors, such as the ALR-specific receptor or c-Met, activating downstream pathways like MAPK/ERK and PI3K/Akt to regulate cell survival and growth. Its recombinant form has garnered interest in preclinical research for liver diseases (e.g., cirrhosis, hepatitis), ischemia-reperfusion injury, and regenerative medicine.
Studies also explore its potential in non-hepatic contexts, such as neuronal protection and cancer therapy, though mechanisms remain under investigation. As a research tool, ALR2278 aids in deciphering ALR's pleiotropic roles, while its therapeutic applications are being optimized for pharmacokinetics and targeted delivery. Safety and efficacy profiles are currently evaluated in experimental models, positioning ALR2278 as a promising candidate for translational biomedicine.
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