| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RYR3 |
| Uniprot No | Q15413 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 4636-4870aa |
| 氨基酸序列 | HYNNFFFAAHLLDIAMGFKTLRTILSSVTHNGKQLVLTVGLLAVVVYLYTVVAFNFFRKFYNKSEDDDEPDMKCDDMMTCYLFHMYVGVRAGGGIGDEIEDPAGDPYEMYRIVFDITFFFFVIVILLAIIQGLIIDAFGELRDQQEQVREDMETKCFICGIGNDYFDTTPHGFETHTLQEHNLANYLFFLMYLINKDETEHTGQESYVWKMYQERCWDFFPAGDCFRKQYEDQLG |
| 预测分子量 | 33.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RYR3重组蛋白的3篇参考文献示例(内容基于学术研究常见方向模拟，仅供参考)：
---
1. **文献名称**: "Expression and functional characterization of recombinant human RYR3 in HEK293 cells"
**作者**: Smith A, et al.
**摘要**: 该研究成功在HEK293细胞中表达了人源RYR3重组蛋白，并通过钙成像技术证实其介导的钙离子释放功能，揭示了RYR3在神经元钙信号调控中的潜在作用。
2. **文献名称**: "Cryo-EM structure of the recombinant RYR3 channel in closed state"
**作者**: Chen L, et al.
**摘要**: 利用冷冻电镜技术解析了重组RYR3蛋白的闭合态三维结构，揭示了其与RYR1/2亚型的关键结构差异，为亚型特异性调控机制提供了分子基础。
3. **文献名称**: "Role of RYR3 recombinant protein in cancer cell migration via calcium signaling"
**作者**: Tanaka K, et al.
**摘要**: 通过体外重组RYR3蛋白实验，证明其在癌细胞中通过调控局部钙震荡促进细胞迁移，提示RYR3可能成为癌症治疗的潜在靶点。
---
*注：上述文献为示例性内容，实际研究中请通过PubMed或Web of Science等数据库检索真实发表的论文。*
**Background of RYR3 Recombinant Protein**
The ryanodine receptor 3 (RYR3) is a member of the ryanodine receptor (RYR) family, a class of intracellular calcium release channels critical for regulating cellular calcium signaling. RYR3. encoded by the *RYR3* gene in humans, is one of three isoforms (RYR1. RYR2. RYR3) that mediate calcium efflux from the endoplasmic/sarcoplasmic reticulum into the cytosol. While RYR1 and RYR2 are predominantly expressed in skeletal and cardiac muscle, respectively, RYR3 exhibits broader tissue distribution, including the brain, smooth muscle, and epithelial cells, and is implicated in diverse physiological processes such as neuronal plasticity, muscle contraction, and cell proliferation.
RYR3 is structurally characterized by a large homotetrameric assembly (~2.2 MDa) with each subunit containing transmembrane domains for channel gating and cytoplasmic regions that interact with modulators like calcium, ATP, and accessory proteins (e.g., calmodulin). Its activation is tightly regulated by calcium-induced calcium release (CICR) and redox-sensitive mechanisms. Dysregulation of RYR3 has been linked to pathologies such as neurodegenerative disorders, cardiovascular diseases, and cancer, though its precise roles remain less defined compared to RYR1/RYR2.
Recombinant RYR3 proteins are engineered in vitro using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to study its structure, function, and interactions. These proteins often include affinity tags (e.g., His-tag) for purification and are used in biochemical assays, structural studies (cryo-EM), or drug screening. Research on RYR3 recombinant proteins aims to elucidate its unique regulatory mechanisms, isoform-specific contributions to calcium signaling, and potential as a therapeutic target. Advances in recombinant technology continue to enhance our understanding of RYR3's role in health and disease.
×
