| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF175 |
| Uniprot No | Q9Y473 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-711aa |
| 氨基酸序列 | MPADVNLSQKPQVLGPEKQDGSCEASVSFEDVTVDFSREEWQQLDPAQRCLYRDVMLELYSHLFAVGYHIPNPEVIFRMLKEKEPRVEEAEVSHQRCQEREFGLEIPQKEISKKASFQKDMVGEFTRDGSWCSILEELRLDADRTKKDEQNQIQPMSHSAFFNKKTLNTESNCEYKDPGKMIRTRPHLASSQKQPQKCCLFTESLKLNLEVNGQNESNDTEQLDDVVGSGQLFSHSSSDACSKNIHTGETFCKGNQCRKVCGHKQSLKQHQIHTQKKPDGCSECGGSFTQKSHLFAQQRIHSVGNLHECGKCGKAFMPQLKLSVYLTDHTGDIPCICKECGKVFIQRSELLTHQKTHTRKKPYKCHDCGKAFFQMLSLFRHQRTHSREKLYECSECGKGFSQNSTLIIHQKIHTGERQYACSECGKAFTQKSTLSLHQRIHSGQKSYVCIECGQAFIQKAHLIVHQRSHTGEKPYQCHNCGKSFISKSQLDIHHRIHTGEKPYECSDCGKTFTQKSHLNIHQKIHTGERHHVCSECGKAFNQKSILSMHQRIHTGEKPYKCSECGKAFTSKSQFKEHQRIHTGEKPYVCTECGKAFNGRSNFHKHQITHTRERPFVCYKCGKAFVQKSELITHQRTHMGEKPYECLDCGKSFSKKPQLKVHQRIHTGERPYVCSECGKAFNNRSNFNKHQTTHTRDKSYKCSYSVKGFTKQ |
| 预测分子量 | 97.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF175重组蛋白的虚构参考文献示例(仅供格式参考，实际文献需通过学术数据库检索)：
1. **文献名称**：*Cloning and Functional Characterization of Recombinant ZNF175 Protein in Gene Regulation*
**作者**：Li, X.; Zhang, Y.; Wang, H.
**摘要**：本研究成功克隆并表达了ZNF175重组蛋白，通过体外实验验证其结合特定DNA序列的能力，揭示了ZNF175在调控肿瘤抑制基因表达中的潜在作用。
2. **文献名称**：*Structural Analysis of ZNF175 Recombinant Protein and Its Interaction with Chromatin Modifiers*
**作者**：Chen, J.; Liu, R.; Zhou, M.
**摘要**：利用X射线晶体学解析了ZNF175重组蛋白的三维结构，发现其锌指结构域与组蛋白去乙酰化酶(HDACs)存在相互作用，提示其在表观遗传调控中的功能。
3. **文献名称**：*ZNF175 Recombinant Protein Suppresses Viral Replication via Targeting Promoter Elements*
**作者**：Kim, S.; Park, J.; Lee, T.
**摘要**：证明ZNF175重组蛋白可通过结合人类巨细胞病毒(HCMV)启动子区域抑制病毒复制，为抗病毒治疗提供了新靶点。
4. **文献名称**：*Development of a ZNF175-Based Recombinant Protein for Epigenetic Editing Applications*
**作者**：Smith, A.; Johnson, B.; Davis, K.
**摘要**：构建了ZNF175重组蛋白与CRISPR/dCas9的融合系统，成功定向编辑特定基因位点的表观遗传标记，展示了其在基因治疗中的潜力。
**注意**：以上文献为模拟示例，实际研究需查阅PubMed、Web of Science等平台获取真实数据(可用关键词：ZNF175 recombinant protein, zinc finger protein 175. gene regulation)。
ZNF175 is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (ZFP) family, which represents one of the largest groups of transcription regulators in humans. These proteins are characterized by tandem arrays of C2H2-type zinc finger motifs that mediate sequence-specific DNA binding, coupled with a KRAB domain at the N-terminus known to recruit epigenetic modifiers like heterochromatin protein 1 (HP1) and histone methyltransferases. This structural configuration enables ZNF175 to function as a transcriptional repressor, potentially silencing target genes involved in developmental processes or cellular homeostasis.
The ZNF175 gene is located on chromosome 19q13.43. a region associated with evolutionary diversification of primate-specific zinc finger genes. While its precise biological role remains understudied, emerging evidence suggests it may contribute to germline development, stem cell differentiation, or tumor suppression. Dysregulation of KRAB-ZFPs, including ZNF175. has been implicated in cancers and neurological disorders, likely through aberrant chromatin remodeling.
Recombinant ZNF175 protein is typically produced in Escherichia coli or mammalian expression systems for functional studies. Its purified form, often tagged with affinity markers like His or FLAG, facilitates in vitro assays such as electrophoretic mobility shift assays (EMSAs) to map DNA-binding sites or chromatin immunoprecipitation (ChIP) to identify genomic targets. Researchers also employ it to explore interactions with co-repressors or to model gene regulatory networks. Current interest in ZNF175 stems from its potential as a tool for synthetic biology or gene editing, leveraging its programmable DNA-binding domains for engineered transcriptional control. However, comprehensive mechanistic insights await further investigation.
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