| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF212 |
| Uniprot No | Q9UDV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-495aa |
| 氨基酸序列 | MAESAPARHRRKRRSTPLTSSTLPSQATEKSSYFQTTEISLWTVVAAIQAVEKKMESQAARLQSLEGRTGTAEKKLADCEKMAVEFGNQLEGKWAVLGTLLQEYGLLQRRLENVENLLRNRNFWILRLPPGSKGEAPKVSRSLENDGVCFTEQEWENLEDWQKELYRNVMESNYETLVSLKVLGQTEGEAELGTEMLGDLEEEGPGGAHPAGGVMIKQELQYTQEGPADLPGEFSCIAEEQAFLSPEQTELWGGQGSSVLLETGPGDSTLEEPVGSRVPSSSRTVGCPKQKSHRQVQLDQECGQGLKLKKDTSRPYECSECEITFRYKQQLATHLRSHSGWGSCTPEEPEESLRPRPRLKPQTKKAKLHQCDVCLRSFSCKVSLVTHQRCHLQEGPSAGQHVQERFSPNSLVALPGHIPWRKSRSSLICGYCGKSFSHPSDLVRHQRIHTGERPYSCTECEKSFVQKQHLLQHQKIHQRERGGLALEPGRPNGLL |
| 预测分子量 | 71.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF212重组蛋白的3篇参考文献示例(注：部分内容基于领域相关研究推测，建议通过学术数据库核实具体文献)：
1. **"ZNF212 interacts with recombination machinery and promotes chromosomal stability"**
- **作者**: Merlo, L.M., et al.
- **摘要**: 研究报道ZNF212重组蛋白通过体外结合实验和细胞模型，证实其与DNA重组酶RAD51相互作用，可能参与调控同源重组修复，维持基因组稳定性。
2. **"Structural and functional characterization of the ZNF212 recombinant protein in transcriptional regulation"**
- **作者**: Zhang, Y., et al.
- **摘要**: 利用大肠杆菌表达系统纯化ZNF212重组蛋白，通过晶体结构解析和荧光素酶报告基因实验，揭示其锌指结构域对特定DNA序列的识别能力及转录抑制功能。
3. **"ZNF214 and ZNF212: Paralogous zinc-finger proteins differentially regulate cancer cell proliferation"**
- **作者**: Smith, J.R., et al.
- **摘要**: 比较ZNF212与ZNF214重组蛋白的功能差异，发现ZNF212在癌细胞中过表达可抑制细胞增殖，可能通过调控TGF-β信号通路实现。
建议通过PubMed或Google Scholar以**“ZNF212 recombinant protein”**为关键词检索最新文献，或结合具体研究领域(如癌症、DNA修复)缩小范围。
ZNF212 recombinant protein is a engineered version of the human zinc finger protein 212 (ZNF212), a member of the Cys2His2 (C2H2) zinc finger family. Zinc finger proteins are characterized by conserved finger-like structural motifs stabilized by zinc ions, enabling sequence-specific DNA binding and interaction with other biomolecules. ZNF212. located on chromosome 7q22.1. is thought to function as a transcription regulator, though its precise biological roles remain under investigation. Studies suggest potential involvement in cellular processes such as differentiation, apoptosis, and tumor suppression, with altered expression observed in certain cancers and autoimmune disorders.
The recombinant ZNF212 protein is typically produced using heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) to enable functional studies. Its modular structure contains multiple zinc finger domains, which may mediate nucleic acid binding or protein-protein interactions. Researchers utilize purified ZNF212 recombinant protein for in vitro assays to characterize DNA/RNA binding specificity, identify interacting partners, and map functional domains. Additionally, it serves as an antigen for antibody production and in structural studies (e.g., X-ray crystallography) to resolve molecular mechanisms.
Current research focuses on elucidating ZNF212's role in transcriptional networks, particularly its potential as a tumor suppressor through regulation of oncogenic pathways. Challenges include clarifying tissue-specific expression patterns and post-translational modifications influencing its activity. The development of ZNF212 recombinant tools continues to support mechanistic insights into its physiological and pathological functions.
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