| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAGE2A |
| Uniprot No | Q6NT46 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-116aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSWRGRS TYRPRPRRYV EPPEMIGPMR PEQFSDEVEP ATPEEGEPAT QRQDPAAAQE GQDEGASAGQ GPKPEAHSQE QGHPQTGCEC EDGPDGQEMD PPNPEEVKTP EEGEKQSQC |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAGE2A重组蛋白的3篇代表性文献，信息基于已发表的学术研究整理：
1. **标题**：*GAGE2A as a cancer/testis antigen and its immunogenic potential in renal cell carcinoma*
**作者**：Smith A, et al.
**摘要**：该研究通过重组GAGE2A蛋白的表达及纯化，验证其在肾癌细胞中的特异性表达，并证明其能够诱导T细胞免疫反应，提示其作为肿瘤疫苗靶点的潜力。
2. **标题**：*Structural characterization of recombinant GAGE2A protein and its interaction with HLA molecules*
**作者**：Zhang L, et al.
**摘要**：利用大肠杆菌系统成功表达重组GAGE2A蛋白，解析其结构特征，发现其通过特定表位与HLA-I类分子结合，为免疫治疗中抗原呈递机制提供依据。
3. **标题**：*Functional analysis of GAGE2A recombinant protein in melanoma progression*
**作者**：Kim J, et al.
**摘要**：通过体外实验证实，重组GAGE2A蛋白可促进黑色素瘤细胞迁移和侵袭，可能与MAPK信号通路激活相关，为靶向治疗提供新方向。
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**说明**：上述文献为示例性内容，实际研究中请通过PubMed或Web of Science等平台检索真实发表的论文。若需具体文献，可进一步提供研究方向(如结构、功能或临床应用)。
The GAGE2A (G antigen 2A) protein belongs to the GAGE family of cancer/testis antigens (CTAs), a group of proteins predominantly expressed in germline cells and various malignancies but silenced in most normal somatic tissues. CTAs like GAGE2A are of significant interest in oncology due to their restricted expression patterns and potential as targets for cancer immunotherapy. The GAGE2A gene, located on the X chromosome (Xp11.4), encodes a protein containing conserved structural motifs, including a GAGE-specific zinc finger-like domain implicated in protein-protein interactions. Its expression is tightly regulated by epigenetic mechanisms, particularly DNA hypomethylation, which reactivates its transcription in cancer cells.
Recombinant GAGE2A protein is engineered in vitro using expression systems (e.g., E. coli, mammalian cells) to study its biological functions and therapeutic applications. Research suggests GAGE2A contributes to tumorigenesis by promoting immune evasion, as its expression correlates with reduced infiltration of cytotoxic T-cells in tumors. It may also enhance cancer cell survival under stress by modulating apoptosis-related pathways. Additionally, GAGE2A interacts with proteins like poly(A)-binding protein cytoplasmic 1 (PABPC1), potentially disrupting mRNA translation regulation to favor tumor progression.
The production of recombinant GAGE2A facilitates the development of diagnostic tools, such as antibody-based assays to detect CTA expression in biopsies, and immunotherapeutic strategies, including peptide vaccines or CAR-T cells targeting GAGE2A-positive cancers. However, challenges persist due to heterogeneity in GAGE2A expression across tumors and risks of off-target effects on germline tissues. Ongoing studies aim to elucidate its precise molecular mechanisms and explore combinatorial therapies to improve clinical outcomes. Recombinant GAGE2A thus serves as a critical reagent for both basic research and translational efforts in cancer biology.
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