| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNG13 |
| Uniprot No | Q9P2W3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-64aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEEWDVP QMKKEVESLK YQLAFQREMA SKTIPELLKW IEDGIPKDPF LNPDLMKNNP WVEKGKC |
| 预测分子量 | 10 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNG13重组蛋白的3篇代表性文献摘要示例(内容基于公开研究概括，非真实文献)：
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1. **标题**：*Structural characterization of recombinant human GNG13 protein in complex with Gβ1*
**作者**：Smith A, et al.
**摘要**：研究通过昆虫细胞表达系统成功获得重组GNG13与Gβ1的复合体蛋白，并利用X射线晶体学解析其三维结构，揭示了GNG13特异性结合Gβ1的关键结构域，为G蛋白信号转导机制提供了新见解。
2. **标题**：*Expression and functional analysis of GNG13 recombinant protein in breast cancer cells*
**作者**：Li X, et al.
**摘要**：构建了哺乳动物细胞表达的重组GNG13蛋白，发现其过表达可抑制乳腺癌细胞迁移，并通过蛋白质相互作用实验证实GNG13通过调控MAPK通路发挥抑癌作用。
3. **标题**：*Optimization of recombinant GNG13 production in E. coli for biochemical studies*
**作者**：Yamamoto K, et al.
**摘要**：开发了一种高效的大肠杆菌重组表达体系，通过密码子优化和纯化策略获得高纯度GNG13蛋白，并验证其与Gα亚基的动态结合能力，为体外信号通路研究提供了工具。
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以上内容综合了结构解析、功能研究和表达优化等方向，涵盖重组蛋白的关键应用场景。
**Background of GNG13 Recombinant Protein**
GNG13 (G Protein Subunit Gamma 13) is a member of the gamma subunit family within heterotrimeric G proteins, which are critical for transmitting intracellular signals from G protein-coupled receptors (GPCRs) to downstream effectors. These subunits, along with alpha and beta subunits, regulate diverse physiological processes, including sensory perception, neurotransmission, and cell proliferation. GNG13 is notably expressed in specific tissues, such as the olfactory epithelium and certain cancer cells, suggesting specialized roles in sensory signaling and oncogenesis.
Recombinant GNG13 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. Its production enables detailed study of GNG13's structure, interactions (e.g., with Gβ subunits or GPCRs), and regulatory mechanisms. Researchers utilize recombinant GNG13 to explore its role in signaling pathways, particularly in olfactory transduction, where it may contribute to odorant receptor specificity, or in cancer, where aberrant expression correlates with tumor progression.
Structural studies of recombinant GNG13 highlight its conserved gamma subunit fold, which facilitates binding to Gβ partners. However, post-translational modifications (e.g., lipid anchoring) critical for membrane localization in native contexts may be absent in recombinant forms, requiring experimental optimization.
Current applications include drug discovery targeting GPCR pathways, mechanistic studies of sensory disorders, and cancer biomarker development. Challenges remain in understanding tissue-specific functions and isoform diversity. Overall, recombinant GNG13 serves as a vital tool for dissecting G protein signaling complexity and its implications in health and disease.
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