| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNGT1 |
| Uniprot No | P63211 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-71aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPVINIE DLTEKDKLKM EVDQLKKEVT LERMLVSKCC EEVRDYVEER SGEDPLVKGI PEDKNPFKEL KGGC |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNGT1重组蛋白的示例参考文献(内容为模拟示例，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"Cloning and functional characterization of human GNGT1: A retina-specific G protein γ-subunit"*
**作者**: Smith A, et al.
**摘要**: 本研究成功克隆并表达了人源GNGT1重组蛋白，发现其在视网膜光信号转导通路中特异性表达，并证实其与视杆细胞G蛋白α亚基(GNAT1)的相互作用，为光信号传导机制提供了新见解。
2. **文献名称**: *"Structural analysis of recombinant GNGT1 in complex with Gβ subunits"*
**作者**: Lee J, et al.
**摘要**: 通过大肠杆菌表达系统纯化GNGT1重组蛋白，结合X射线晶体学解析其与Gβ亚基的复合物结构，揭示了Gγ亚基在G蛋白异源三聚体组装中的关键结合位点。
3. **文献名称**: *"GNGT1 knockout mice exhibit impaired phototransduction and retinal degeneration"*
**作者**: Chen L, et al.
**摘要**: 利用CRISPR技术构建GNGT1基因敲除小鼠模型，发现其视网膜光响应能力显著下降，重组GNGT1蛋白回补实验证实其在维持光感受器细胞功能中的必要性。
4. **文献名称**: *"High-yield production of bioactive GNGT1 in insect cells for drug screening"*
**作者**: Wang Y, et al.
**摘要**: 报道了一种基于杆状病毒-昆虫细胞系统的GNGT1重组蛋白高效表达方法，纯化蛋白可用于筛选靶向G蛋白信号通路的小分子抑制剂，为药物开发提供工具。
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**注意**：以上文献为模拟示例，实际研究请通过PubMed、Web of Science或Google Scholar等平台检索关键词“GNGT1 recombinant protein”或“GNGT1 function”获取最新论文。
**Background of GNGT1 Recombinant Protein**
GNGT1 (G Protein Subunit Gamma Transducin 1) is a gene encoding the gamma subunit of the heterotrimeric G-protein complex, which plays a critical role in intracellular signal transduction. G-proteins consist of α, β, and γ subunits, and their activation by G-protein-coupled receptors (GPCRs) triggers downstream signaling cascades regulating diverse physiological processes. The GNGT1-encoded gamma subunit is particularly notable for its role in the visual phototransduction pathway. In retinal photoreceptor cells, it partners with the beta subunit (GNB1) and the alpha subunit of transducin (GNAT1) to form the Gt protein complex, essential for converting light signals into electrochemical responses.
Recombinant GNGT1 protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, to study its structure, function, and interactions. Its recombinant form enables researchers to explore molecular mechanisms underlying visual signaling, GPCR-mediated pathways, and potential dysregulation in diseases. For instance, mutations in G-protein subunits have been linked to retinal degeneration and other vision disorders. Additionally, GNGT1 may interact with non-visual GPCRs, suggesting broader regulatory roles in cellular processes like cell growth or immune responses.
Studies using recombinant GNGT1 have also contributed to drug discovery, particularly in designing therapies targeting G-protein-related pathologies. Its application extends to *in vitro* assays, antibody production, and structural biology (e.g., crystallography). Furthermore, aberrant expression of GNGT1 has been observed in certain cancers, highlighting its potential as a biomarker or therapeutic target. Overall, recombinant GNGT1 serves as a vital tool for dissecting G-protein biology and advancing translational research in vision science, oncology, and beyond.
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