| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNG4 |
| Uniprot No | P50150 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-72aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMKEGMSNNSTTSISQARKAVEQLKMEA CMDRVKVSQAAADLLAYCEAHVREDPLIIPVPASENPFREKKFFC |
| 预测分子量 | 10 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇关于GNG4重组蛋白的模拟参考文献示例(请注意，这些文献为虚构内容，建议通过PubMed或Google Scholar查询真实文献)：
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1. **文献名称**：*Structural characterization of recombinant GNG4 protein and its role in G-protein signaling*
**作者**：Chen L, et al.
**摘要**：本研究通过原核表达系统成功重组表达并纯化了人源GNG4蛋白，利用X射线晶体学解析了其三维结构。研究发现GNG4通过与Gβ亚基特异性结合，调控G蛋白偶联受体(GPCR)下游信号通路，并揭示了其在肿瘤细胞迁移中的潜在作用。
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2. **文献名称**：*Functional analysis of GNG4 in neural development using recombinant protein models*
**作者**：Kim S, et al.
**摘要**：通过体外重组表达GNG4蛋白，结合小鼠神经元模型，发现GNG4在轴突导向和突触形成中起关键作用。研究证实GNG4与Semaphorin信号通路相互作用，可能为神经系统疾病的治疗提供靶点。
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3. **文献名称**：*Recombinant GNG4 as a biomarker for colorectal cancer progression*
**作者**：Wang Y, et al.
**摘要**：该研究通过重组GNG4蛋白制备抗体，分析其在结直肠癌组织中的表达水平。结果表明，GNG4高表达与肿瘤转移和患者预后不良显著相关，提示其可作为潜在的诊断标志物。
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4. **文献名称**：*Interaction between GNG4 and oncogenic KRAS in pancreatic cancer cells*
**作者**：Rodriguez M, et al.
**摘要**：利用重组GNG4蛋白进行体外结合实验，发现GNG4与KRAS蛋白存在直接互作，并通过调控MAPK信号通路促进胰腺癌细胞增殖。研究为靶向G蛋白信号的抗癌策略提供了依据。
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**注意**：以上文献信息为模拟生成，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
G protein subunit gamma 4 (GNG4) is a member of the heterotrimeric G protein gamma subunit family, which plays critical roles in cellular signal transduction. These proteins form stable complexes with Gβ subunits, creating functional Gβγ dimers that interact with G protein-coupled receptors (GPCRs), effectors, and regulators. GNG4 is encoded by the GNG4 gene located on human chromosome 16 and is expressed in various tissues, including the brain, heart, and immune cells. Its specific functions remain less characterized compared to other Gγ subunits, but emerging studies suggest involvement in neurological processes, cardiovascular regulation, and cancer progression. For instance, GNG4 has been linked to synaptic plasticity modulation and dopamine receptor signaling in the central nervous system. Dysregulation of GNG4 expression has been observed in gliomas, breast cancer, and neurodevelopmental disorders, highlighting its potential role in disease mechanisms.
Recombinant GNG4 protein is engineered in vitro using expression systems like E. coli or mammalian cells, often fused with tags (e.g., His-tag) for purification and detection. This protein serves as a vital tool for studying Gβγ dimer assembly, GPCR signaling cascades, and downstream effectors such as ion channels or adenylyl cyclases. Researchers utilize recombinant GNG4 to explore its structural interactions with Gβ subunits, map binding interfaces, and screen for small-molecule modulators targeting Gβγ-mediated pathways. Its applications extend to drug discovery, particularly in diseases where aberrant GPCR signaling is implicated, including cancer metastasis, neurological disorders, and cardiovascular conditions. Ongoing research aims to clarify tissue-specific roles of GNG4 and its potential as a therapeutic target or biomarker.
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