| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NKB |
| Uniprot No | Q9UHF0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-121aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMQSFGAVCKEPQEEVVPGGGRSKRDPDLYQ LLQRLFKSHSSLEGLLKALSQASTDPKESTSPEKRDMHDFFVGLMGKRSV QPDSPTDVNQENVPSFGILKYPPRAE |
| 预测分子量 | 14 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于NKB(神经激肽B)重组蛋白研究的示例文献(内容为模拟概括，建议通过学术数据库检索真实文献)：
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1. **文献名称**: *"Expression and Purification of Recombinant Neurokinin B in Escherichia coli"*
**作者**: Zhang Y, et al.
**摘要**: 本研究建立了在大肠杆菌中高效表达NKB重组蛋白的工艺，通过优化密码子和纯化步骤获得高纯度产物，并验证了其与NK3受体的体外结合活性。
2. **文献名称**: *"Structural Analysis of Recombinant Neurokinin B by NMR Spectroscopy"*
**作者**: Smith J, et al.
**摘要**: 利用核磁共振技术解析了重组NKB的三维结构，揭示了其与受体结合的活性区域，为基于结构的药物设计提供了依据。
3. **文献名称**: *"Role of Recombinant NKB in Modulating Hypothalamic Neuronal Activity"*
**作者**: Lee S, et al.
**摘要**: 通过体外实验证明重组NKB蛋白可特异性激活下丘脑神经元中的NK3受体通路，提示其在生殖内分泌调控中的潜在作用。
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**备注**：以上文献信息为示例性质，具体研究请参考PubMed、ScienceDirect等平台检索关键词"recombinant neurokinin B"或"NKB protein expression"。
**Background of NKB Recombinant Protein**
Neurokinin B (NKB), encoded by the *TAC3* gene in humans, is a neuropeptide belonging to the tachykinin family, which also includes substance P and neurokinin A. It binds preferentially to the neurokinin-3 receptor (NK3R), a G protein-coupled receptor (GPCR), and plays critical roles in regulating reproductive physiology, energy homeostasis, and neuroendocrine signaling. NKB gained prominence due to its involvement in the hypothalamic control of gonadotropin-releasing hormone (GnRH) secretion, particularly through interactions with the Kisspeptin system. Mutations in *TAC3* or its receptor (*TACR3*) are linked to congenital hypogonadotropic hypogonadism, underscoring its importance in puberty and fertility.
Recombinant NKB protein is produced using genetic engineering techniques, typically through expression in bacterial (e.g., *E. coli*) or mammalian cell systems. The recombinant form retains the biological activity of endogenous NKB, enabling researchers to study its structure-function relationships, receptor interactions, and downstream signaling pathways *in vitro* or *in vivo*. Its applications span basic research—such as elucidating reproductive neuroendocrinology—and drug discovery, where it serves as a tool for screening NK3R-targeted therapeutics.
Interest in NKB has expanded due to its potential role in metabolic disorders, mood regulation, and cancer. Recombinant NKB is also utilized in diagnostics and biomarker studies, particularly in reproductive health. Despite challenges in peptide stability and delivery, advancements in protein engineering and formulation continue to enhance its utility. Overall, NKB recombinant protein represents a vital resource for understanding tachykinin biology and developing interventions for related disorders.
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