| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | USE1; HOYS7 |
| WB Predicted band size | 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human UBE2Z |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于UBE2Z抗体的3篇示例参考文献(内容为虚构，仅作格式参考)：
1. **文献名称**: "UBE2Z-mediated ubiquitination regulates NF-κB signaling in cancer"
**作者**: Smith A, et al. (2015)
**摘要**: 本研究揭示了UBE2Z通过调控IκBα的泛素化影响NF-κB信号通路，利用特异性UBE2Z抗体证实其在多种癌细胞系中的高表达，并证明其促进肿瘤生长和化疗抵抗的机制。
2. **文献名称**: "Development of a monoclonal antibody against UBE2Z for functional proteomics analysis"
**作者**: Chen L, et al. (2017)
**摘要**: 报道了一种新型UBE2Z单克隆抗体的开发与验证，该抗体通过ELISA和免疫共沉淀(Co-IP)验证高特异性，应用于组织微阵列分析显示UBE2Z在肝癌组织中异常上调。
3. **文献名称**: "UBE2Z interacts with BRCA1 and modulates DNA repair pathways"
**作者**: Johnson R, et al. (2020)
**摘要**: 利用UBE2Z抗体进行免疫荧光和蛋白质互作研究，发现UBE2Z与BRCA1复合物协同参与DNA损伤修复，其缺失导致同源重组修复缺陷，提示其在基因组稳定性中的关键作用。
**备注**：以上文献为示例，实际引用请通过PubMed/Google Scholar等平台检索真实文献。
UBE2Z (Ubiquitin-Conjugating Enzyme E2 Z) is a member of the E2 ubiquitin-conjugating enzyme family, which plays a critical role in the ubiquitin-proteasome system (UPS). This system regulates protein degradation, post-translational modifications, and various cellular processes, including DNA repair, cell cycle progression, and stress responses. UBE2Z specifically catalyzes the transfer of ubiquitin to substrate proteins, often working in tandem with E3 ubiquitin ligases to determine substrate specificity. It is involved in forming Lys-63-linked polyubiquitin chains, which are associated with non-proteolytic functions like signal transduction and protein trafficking.
Antibodies targeting UBE2Z are essential tools for studying its expression, localization, and functional interactions. These antibodies are typically developed using immunogens derived from conserved regions of the human UBE2Z protein and are validated for applications such as Western blotting, immunofluorescence, immunoprecipitation, and immunohistochemistry. Researchers employ UBE2Z antibodies to investigate its role in diseases linked to UPS dysregulation, including cancers, neurodegenerative disorders, and autoimmune conditions. For example, aberrant UBE2Z activity has been implicated in tumor progression and chemoresistance, making it a potential therapeutic target. Reliable UBE2Z antibodies help elucidate its molecular mechanisms and contribute to biomarker discovery or drug development efforts.
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