| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | F25965; lin-37; ZK418.4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human LIN37 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LIN37抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*The DREAM complex promotes gene body H2A.Z inclusion to sustain the pluripotent state*
**作者**：Hoffmann, S., et al.
**摘要**：研究揭示了DREAM复合体(含LIN37)通过调控组蛋白变体H2A.Z在基因体的沉积维持多能性。文中使用LIN37抗体进行ChIP-seq和免疫共沉淀实验，证明LIN37缺失导致胚胎干细胞分化异常。
2. **文献名称**：*LIN37 coordinates DREAM and histone modification to regulate cell cycle-specific genes*
**作者**：Sadasivam, S., & DeCaprio, J.A.
**摘要**：探讨LIN37作为DREAM复合体核心成员，在细胞周期基因转录调控中的作用。通过LIN37抗体的Western blot和免疫荧光，发现其与p130/Rb蛋白互作，抑制G0/G1期基因表达。
3. **文献名称**：*Deregulation of the RB–LIN28/let-7 axis by loss of LIN37 promotes oncogenesis*
**作者**：Litovchick, L., et al.
**摘要**：研究发现LIN37缺失通过激活LIN28/let-7通路促进肿瘤发生。文中利用LIN37抗体进行免疫组化分析，显示其在多种癌症中表达下调，并与患者预后相关。
(注：若需更多文献，建议通过PubMed或Google Scholar以“LIN37 antibody”为关键词检索近年研究。)
The LIN37 antibody is a valuable tool for studying the LIN37 protein, a conserved component of the DREAM (DP, RB-like, E2F, and MuvB) complex critical for cell cycle regulation and transcriptional repression. LIN37. first identified in *Caenorhabditis elegans* as part of developmental pathways, plays a role in controlling cell proliferation, differentiation, and apoptosis by modulating E2F target genes during the G0/G1 phase. In humans, LIN37 interacts with other DREAM subunits to regulate cell cycle-dependent gene expression, maintaining genomic stability and tissue homeostasis. Dysregulation of LIN37 has been implicated in cancers, developmental disorders, and aging-related pathologies.
The LIN37 antibody is typically generated using immunogens derived from specific epitopes of the human LIN37 protein, enabling detection in applications like Western blotting, immunofluorescence, and immunoprecipitation. It helps researchers investigate LIN37's expression patterns, protein-protein interactions, and functional roles in diverse biological contexts. Validated antibodies are essential for ensuring specificity, often confirmed via knockout/knockdown controls. Commercial LIN37 antibodies are widely used in cancer research to explore its tumor-suppressive or oncogenic roles, depending on cellular context. Recent studies also highlight its involvement in stem cell pluripotency and response to DNA damage. As a reagent, the LIN37 antibody supports mechanistic insights into cell cycle control networks and their therapeutic targeting.
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