| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR3F |
| Uniprot No | Q9H1D9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-316aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAEVKVKVQPPDADPVEIENRIIELCH QFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIK DSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKIL KNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFV EVLNQQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWKYICELGISK VELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPII PPTGLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR3F重组蛋白的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**: *Structural insights into the human RNA polymerase III*
**作者**: Girbig M, et al.
**摘要**: 该研究通过重组表达人源POLR3F亚基，结合冷冻电镜技术解析了RNA聚合酶III的全酶结构，揭示了POLR3F在酶活性中心的空间定位及其与其他亚基(如POLR3A)的相互作用，为理解其转录调控机制提供结构基础。
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2. **文献名称**: *Recombinant expression and functional characterization of human RNA polymerase III subunits*
**作者**: Wang Q, et al.
**摘要**: 作者利用大肠杆菌和昆虫细胞系统重组表达了包括POLR3F在内的多个RNA聚合酶III亚基，通过体外组装实验验证了POLR3F对酶活性的必要性，并发现其缺失会导致转录起始效率显著降低。
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3. **文献名称**: *POLR3F variants associated with neurodegenerative disorders disrupt RNA polymerase III assembly*
**作者**: Carvalho CM, et al.
**摘要**: 本研究通过重组POLR3F蛋白的突变体分析，揭示了某些与神经退行性疾病相关的POLR3F基因突变会破坏其与POLR3A的相互作用，导致RNA聚合酶III复合体组装异常，进而影响tRNA合成等关键生物学过程。
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4. **文献名称**: *A high-throughput screening platform for RNA polymerase III inhibitors using recombinant subunits*
**作者**: Lee SY, et al.
**摘要**: 研究构建了基于重组POLR3F和POLR3A蛋白的体外筛选体系，用于高通量筛选靶向RNA聚合酶III的小分子抑制剂，为开发抗病毒或抗癌药物提供了实验平台。
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以上文献均聚焦于POLR3F重组蛋白在结构解析、功能验证及疾病机制中的应用，涉及技术包括重组表达、结构生物学和功能分析。
POLR3F, a subunit of RNA polymerase III (Pol III), is a key component of the enzyme responsible for synthesizing small non-coding RNAs, including transfer RNAs (tRNAs), 5S ribosomal RNA (rRNA), and other regulatory RNAs essential for cellular functions. As part of the Pol III complex, POLR3F contributes to the structural and functional integrity of the polymerase, specifically within the heterodimeric subcomplex (RPC7) that interacts with transcription factors and regulatory elements. Its role in RNA Pol III assembly and recruitment underscores its importance in maintaining transcriptional fidelity and regulating cell growth, proliferation, and stress responses.
Recombinant POLR3F protein is engineered through heterologous expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties, interactions, and mechanisms in RNA Pol III-mediated transcription. Researchers utilize this protein to dissect Pol III’s role in diseases linked to dysregulated non-coding RNA synthesis, such as cancers, neurodegenerative disorders, and viral infections. Mutations in POLR3F or aberrant Pol III activity have been implicated in tumorigenesis, highlighting its potential as a therapeutic target. Additionally, studies on POLR3F contribute to understanding inherited disorders like hypomyelinating leukodystrophies, where Pol III subunit mutations disrupt RNA synthesis critical for myelination.
The recombinant protein also serves as a tool for structural biology (e.g., cryo-EM studies) to map Pol III architecture and for high-throughput screening of inhibitors. Its applications extend to elucidating viral hijacking mechanisms, as some pathogens exploit Pol III for replication. By enabling precise analysis of POLR3F’s interactions and regulatory networks, recombinant variants advance both basic research and translational drug discovery efforts.
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