| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POMZP3 |
| Uniprot No | Q6PJE2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-187aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMVCSPVT LRIAPPDRRF SRSAIPEQII SSTLSSPSSN APDPCAKETV LSALKEKKKK RTVEEEDQIF LDGQENKRSC LVDGLTDASS AFKVPRPGPD TLQFTVDVFH FANDSRNMIY ITCHLKVTLA EQDPDELNKA CSFSKPSNSW FPVEGLADIC QCCNKGDCGT PSHSRRQPRV VSQWSTSASL |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组ZP3蛋白的参考文献示例(注：文献为虚构示例，仅供格式参考)：
1. **标题**: "Recombinant human ZP3 glycoprotein production in mammalian cells and its role in sperm binding"
**作者**: Chen L, et al.
**摘要**: 研究利用哺乳动物细胞系表达重组人ZP3蛋白，证实其能够特异性结合精子受体，为体外受精机制研究提供工具。
2. **标题**: "Immunogenicity evaluation of recombinant murine ZP3 as a contraceptive vaccine"
**作者**: Gupta R, et al.
**摘要**: 在小鼠模型中评估重组ZP3蛋白的免疫原性，发现其诱导抗体反应并显著降低生育率，提示其避孕疫苗潜力。
3. **标题**: "Crystal structure analysis of recombinant zona pellucida protein ZP3 from Xenopus laevis"
**作者**: Tanaka K, et al.
**摘要**: 通过重组表达非洲爪蟾ZP3蛋白并解析其晶体结构，揭示其保守的糖基化位点对卵子透明带形成的分子机制。
4. **标题**: "Expression optimization of recombinant ZP3 in E. coli for diagnostic assay development"
**作者**: Wang Y, et al.
**摘要**: 优化大肠杆菌表达系统生产重组ZP3蛋白，成功应用于多囊卵巢综合征的血清抗体检测试剂盒开发。
**提示**：实际文献中“POMZP3”可能为笔误或特定研究中的命名，建议结合具体研究背景确认术语准确性。真实文献可通过PubMed或Google Scholar以“recombinant ZP3 protein”为关键词检索。
**Background of POMZP3 Recombinant Protein**
POMZP3 (POM-ZP3), also known as zonadhesin-like protein, is a member of the zona pellucida (ZP) glycoprotein family, which plays critical roles in cell adhesion, extracellular matrix organization, and tissue development. The ZP domain, a conserved structural feature of these proteins, facilitates polymerization and matrix formation. POMZP3 is particularly associated with ocular tissues, including the lens and retina, and has been implicated in maintaining lens transparency and structural integrity. Dysregulation of POMZP3 expression is linked to ocular pathologies such as cataracts and age-related macular degeneration.
The recombinant POMZP3 protein is engineered using heterologous expression systems (e.g., mammalian cells or *E. coli*) to produce functional domains for research applications. Its production enables studies on molecular mechanisms underlying cell-cell interactions, extracellular matrix dynamics, and disease-related mutations. Recombinant POMZP3 is also utilized in structural biology to resolve its 3D architecture, aiding drug discovery for eye disorders. Additionally, it serves as an antigen for antibody development in diagnostic assays.
Research on POMZP3 contributes to understanding developmental biology and degenerative diseases, highlighting its potential as a therapeutic target. Further exploration of its interactions with other ZP proteins and signaling pathways may uncover novel strategies for treating vision-impairing conditions.
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