| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POP4 |
| Uniprot No | O95707 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-220aa |
| 氨基酸序列 | MKSVIYHALSQKEANDSDVQPSGAQRAEAFVRAFLKRSTPRMSPQAREDQLQRKAVVLEYFTRHKRKEKKKKAKGLSARQRRELRLFDIKPEQQRYSLFLPLHELWKQYIRDLCSGLKPDTQPQMIQAKLLKADLHGAIISVTKSKCPSYVGITGILLQETKHIFKIITKEDRLKVIPKLNCVFTVETDGFISYIYGSKFQLRSSERSAKKFKAKGTIDL |
| 预测分子量 | 41.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POP4重组蛋白研究的3篇示例参考文献(注：内容为模拟生成，非真实文献)：
1. **《Recombinant POP4 protein expression and its role in ribosome biogenesis》**
- 作者：Zhang L, et al.
- 摘要：研究通过大肠杆菌系统表达并纯化重组POP4蛋白，证实其在核糖体RNA前体加工中的内切酶活性，并揭示其与核糖体组装复合物的相互作用机制。
2. **《Structural insights into POP4 recombinant protein and its implications for neurodegenerative diseases》**
- 作者：Kim S, Patel R.
- 摘要：利用X射线晶体学解析重组POP4蛋白的三维结构，发现其底物结合位点突变与异常蛋白聚集相关，为阿尔茨海默病病理研究提供新靶点。
3. **《Optimization of POP4 recombinant production in mammalian cell systems for functional assays》**
- 作者：Gomez F, et al.
- 摘要：开发HEK293细胞中重组POP4的高效表达体系，验证其在体外剪接体激活实验中的功能，为基因治疗载体开发提供技术基础。
**注意事项**：
- POP4(POP4 homolog, ribonuclease P/MRP subunit)通常参与非编码RNA加工，实际文献需通过学术数据库(如PubMed)以"POP4 recombinant"或"POP4 protein"为关键词检索。
- 建议结合具体研究方向(如结构、疾病或生产优化)筛选近年高被引论文。
**Background of POP4 Recombinant Protein**
POP4 (Processing of Precursor 4), also known as RPP29 or POP4. is a key protein component of two evolutionarily conserved ribonucleoprotein complexes: RNase P and RNase MRP. These complexes play essential roles in RNA processing. RNase P is responsible for cleaving the 5'-leader sequences of precursor transfer RNAs (pre-tRNAs) to generate mature tRNAs, while RNase MRP primarily processes ribosomal RNA (rRNA) precursors and regulates mitochondrial DNA replication. POP4 contributes to the structural stability and catalytic activity of these complexes by interacting with RNA subunits and other protein partners.
The recombinant POP4 protein is produced using *in vitro* expression systems (e.g., *E. coli* or mammalian cell cultures) to ensure high purity and functionality for research applications. Its recombinant form enables detailed studies of RNase P/MRP assembly, RNA-protein interactions, and enzymatic mechanisms. Dysregulation of POP4 has been linked to human diseases, including cartilage-hair hypoplasia (CHH), a genetic disorder characterized by skeletal dysplasia and immune dysfunction, highlighting its biological significance.
Research on POP4 recombinant protein aids in elucidating its role in RNA metabolism, cellular homeostasis, and disease pathogenesis. It is also valuable for drug screening and therapeutic development targeting RNA-processing disorders. Structural analyses (e.g., X-ray crystallography, cryo-EM) using recombinant POP4 have advanced understanding of ribozyme-protein coordination, offering insights into ancient RNA-based enzymatic systems.
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