| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Probable ATP-dependent RNA helicase DDX60-like, DEAD box protein 60-like, DDX60L |
| Entrez GeneID | 91351 |
| WB Predicted band size | 197.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DDX60L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1200-1228 amino acids from the C-terminal region of human DDX60L. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DDX60L抗体的3篇代表性文献(内容基于假设性研究，实际文献需进一步验证)：
1. **文献名称**: *"DDX60L as a cytoplasmic antiviral helicase: Role in RIG-I-mediated immune response"*
**作者**: Tanaka Y, et al.
**摘要**: 本研究揭示了DDX60L作为RNA解旋酶在RIG-I信号通路中的抗病毒功能。通过开发特异性DDX60L抗体，作者证实其在病毒感染后与线粒体抗病毒信号蛋白(MAVS)相互作用，并促进I型干扰素产生。
2. **文献名称**: *"Characterization of a monoclonal antibody targeting human DDX60L for immune profiling"*
**作者**: Smith J, et al.
**摘要**: 报道了一种新型抗人DDX60L单克隆抗体的开发与验证。该抗体通过流式细胞术和免疫组化证实了DDX60L在免疫细胞(如树突状细胞)中的特异性表达，并用于研究其在慢性炎症疾病中的表达变化。
3. **文献名称**: *"Functional interplay between DDX60L and viral RNA sensors in zebrafish models"*
**作者**: Chen L, et al.
**摘要**: 利用斑马鱼模型及DDX60L多克隆抗体，研究发现DDX60L通过增强MDA5对病毒RNA的识别能力，调控宿主先天性免疫应答，抗体染色显示其在鱼类免疫组织中高表达。
**备注**：上述文献为示例性质，实际引用需通过PubMed或Google Scholar以“DDX60L antibody”或“DDX60L function”为关键词检索近期论文。若研究较少，可扩展至综述或相关蛋白(如DDX60)的文献。
The DDX60L antibody is a tool developed to study the DEAD-box helicase 60-like (DDX60L) protein, a member of the DEAD-box RNA helicase family involved in RNA metabolism and antiviral immune responses. DDX60L shares homology with DDX60. both functioning as cytosolic sensors or modulators in innate immunity. Research suggests DDX60L interacts with RIG-I-like receptors (RLRs) to enhance antiviral signaling, promoting interferon (IFN) production and restricting viral replication. Its role extends to regulating RNA virus infections, including hepatitis C and dengue virus, by facilitating viral RNA degradation or immune activation.
Antibodies targeting DDX60L are typically generated using recombinant protein fragments or peptide immunogens, validated for specificity in applications like Western blotting, immunofluorescence, and immunoprecipitation. These reagents help elucidate DDX60L’s subcellular localization (e.g., cytoplasmic distribution), expression patterns across tissues, and dynamic changes during infections or inflammatory conditions. Emerging studies explore DDX60L’s potential links to autoimmune diseases and cancer, where dysregulated IFN signaling may contribute to pathogenesis. Despite progress, DDX60L’s exact mechanistic roles and interplay with other helicases remain under investigation. The antibody thus serves as a critical reagent for dissecting its contributions to antiviral defense, RNA processing, and disease pathways.
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