Rabbit Polyclonal NCoA3(Phospho-Ser857) Antibody

The NCoA3 (Phospho-Ser857) antibody is designed to detect the nuclear receptor coactivator 3 (NCoA3. also known as AIB1 or SRC-3) when phosphorylated at serine 857. NCoA3 is a member of the steroid receptor coactivator (SRC) family, which plays a critical role in enhancing transcriptional activity of nuclear receptors and transcription factors involved in cell proliferation, differentiation, and metabolism. Phosphorylation at Ser857. mediated by kinases such as IKKε or MAPK, regulates NCoA3's stability, subcellular localization, and interaction with other transcriptional regulators. This post-translational modification is associated with enhanced coactivator function, promoting oncogenic signaling in hormone-dependent cancers, including breast and prostate cancer. The antibody is commonly used in research to study NCoA3 activation dynamics, particularly in contexts like tumor progression, endocrine resistance, or cellular stress responses. Applications include Western blotting, immunohistochemistry, and immunofluorescence to assess phosphorylation-dependent NCoA3 behavior in cell lines, tissues, or disease models. Its specificity for the phosphorylated form makes it valuable for dissecting signaling pathways that modulate coactivator activity in both physiological and pathological states, offering insights into therapeutic targeting of NCoA3-driven malignancies.