Rabbit Polyclonal NDEL1(Phospho-Thr219) Antibody

The NDEL1 (Nuclear Distribution Element-Like 1) protein plays a critical role in neurodevelopment, particularly in neuronal migration and mitosis. Phosphorylation at threonine 219 (Thr219) is a key post-translational modification that regulates NDEL1's interaction with LIS1 (Lissencephaly-1), a protein essential for microtubule dynamics and dynein motor complex function. This phosphorylation event is mediated by Cdk5 (Cyclin-dependent kinase 5) and is crucial for proper neuronal positioning during cortical development. Antibodies targeting NDEL1 phosphorylated at Thr219 (Phospho-Thr219) are valuable tools for studying cell cycle regulation, neurodevelopmental processes, and diseases linked to neuronal migration defects, such as lissencephaly and schizophrenia. These antibodies enable researchers to detect and quantify the activated form of NDEL1 in various experimental models, including Western blotting, immunofluorescence, and immunohistochemistry. Their specificity helps elucidate spatial-temporal phosphorylation patterns in developing neurons or mitotic cells, providing insights into how dysregulated NDEL1 phosphorylation contributes to pathological conditions. Validation typically involves using phosphorylated peptide competition assays and cell models with manipulated Cdk5 activity to confirm target specificity.