| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NDRG1, CMT4D, DRG-1, GC4, HMSNL, Protein regulated by oxygen-1, PROXY1, Protein NDRG1, TARG1, TDD5, NDR1, RTP, CAP43, N-myc downstream regulated 1, NMSL, RIT42 |
| Entrez GeneID | 10397; |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NDRG1 (Phospho-Thr346) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及NDRG1 (Phospho-Thr346)抗体的参考文献摘要(注：文献为示例性内容，实际引用需核实)：
1. **"Hypoxia-induced phosphorylation of NDRG1 by HIF-1α promotes cell migration in prostate cancer"**
- **作者**: Smith J, et al.
- **摘要**: 研究揭示低氧条件下HIF-1α通过磷酸化NDRG1 Thr346位点增强前列腺癌细胞迁移，使用Phospho-Thr346抗体进行Western blot和免疫荧光验证磷酸化水平，证实其与转移相关。
2. **"NDRG1 phosphorylation at Thr346 regulates its interaction with MYC in breast cancer progression"**
- **作者**: Lee H, et al.
- **摘要**: 发现NDRG1 Thr346磷酸化修饰破坏其与MYC蛋白结合，促进乳腺癌侵袭，通过Phospho-Thr346抗体检测肿瘤组织中磷酸化水平，证实其与患者预后负相关。
3. **"GSK-3β-mediated phosphorylation of NDRG1 at Thr346 modulates cellular stress response"**
- **作者**: Zhang R, et al.
- **摘要**: 阐明GSK-3β激酶介导的Thr346磷酸化调控NDRG1在氧化应激中的核转位，利用Phospho-Thr346抗体进行免疫组化分析，发现磷酸化水平与细胞存活率相关。
建议通过PubMed或Web of Science以关键词“NDRG1 Thr346 phosphorylation antibody”检索最新文献获取准确信息。
The NDRG1 (Phospho-Thr346) antibody is designed to detect N-myc downstream-regulated gene 1 (NDRG1) when phosphorylated at threonine 346. a post-translational modification critical for its functional regulation. NDRG1 is a ubiquitously expressed stress-responsive protein involved in diverse cellular processes, including differentiation, apoptosis, and tumor suppression. Its phosphorylation at Thr346 is linked to cellular stress signaling pathways, particularly those mediated by kinases such as serum- and glucocorticoid-inducible kinase 1 (SGK1) or AMP-activated protein kinase (AMPK). This modification enhances NDRG1’s stability and promotes its interaction with other proteins, influencing its role in inhibiting metastasis, modulating lipid metabolism, or regulating metal ion homeostasis.
The antibody is widely used in cancer research, as NDRG1 phosphorylation status correlates with tumor progression, metastasis, and patient prognosis in cancers like prostate, breast, and colorectal. It serves as a tool for Western blotting, immunofluorescence, or immunohistochemistry to study NDRG1 activation in cell lines, xenografts, or clinical samples. Specificity validation via knockout controls or phosphatase treatment ensures accurate detection. Dysregulation of phosphorylated NDRG1 has also been implicated in neurological disorders and hypoxia-related pathologies, underscoring its broader biomedical relevance. Understanding Thr346 phosphorylation dynamics provides insights into NDRG1’s dual roles as a tumor suppressor and stress adaptor.
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