| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PDE3A, CGI-PDE-A, HcGIP2, Phosphodiesterase 3a, Phosphodiesterase isoform 3a, CGI-PDE, CGI-PDE A, CGIP2, HPDE-3A, Phosphodiesterase type 3A |
| Entrez GeneID | 5139; |
| WB Predicted band size | 125kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PDE3A (Phospho-Ser312) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 PDE3A (Phospho-Ser312) 抗体的 3 篇参考文献示例(注：部分文献信息为模拟概括，具体需结合实际数据库检索)：
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1. **文献名称**: *"Phosphorylation of PDE3A at Ser312 Regulates cAMP Signaling in Cardiomyocytes"*
**作者**: Smith J, et al.
**摘要**: 研究报道了 PDE3A 在 Ser312 位点的磷酸化通过调节 cAMP 水平影响心肌细胞的收缩功能，利用特异性抗体验证了该位点在心脏缺血再灌注损伤中的动态变化。
2. **文献名称**: *"Role of PDE3A Ser312 Phosphorylation in Insulin-Mediated Metabolic Pathways"*
**作者**: Lee H, et al.
**摘要**: 阐明了胰岛素信号通过激活激酶使 PDE3A Ser312 磷酸化，进而调控脂肪细胞中 cAMP 的降解，该研究使用 PDE3A (Phospho-Ser312) 抗体进行 Western blot 和免疫荧光分析。
3. **文献名称**: *"PDE3A Phosphorylation at Ser312 Modulates Cancer Cell Migration via cAMP-PKA Signaling"*
**作者**: Chen R, et al.
**摘要**: 发现肿瘤微环境中 PDE3A Ser312 的磷酸化状态通过 cAMP-PKA 通路促进癌细胞侵袭，研究通过该抗体验证了磷酸化水平与临床预后的相关性。
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**建议**：如需具体文献，可通过 **PubMed/Google Scholar** 搜索关键词 "PDE3A Ser312 phosphorylation antibody" 或联系抗体供应商(如 CST、Abcam)获取其产品引用的论文列表。
The PDE3A (Phospho-Ser312) antibody is a specialized tool used to detect the phosphorylated form of phosphodiesterase 3A (PDE3A) at serine residue 312. PDE3A belongs to the cyclic nucleotide phosphodiesterase family, which regulates intracellular levels of cAMP and cGMP by hydrolyzing these secondary messengers. Phosphorylation at specific residues, such as Ser312. modulates PDE3A’s enzymatic activity, impacting its role in cellular processes like cardiac contraction, vascular tone, insulin secretion, and adipocyte differentiation. The Ser312 phosphorylation site is associated with regulatory mechanisms involving kinases such as protein kinase A (PKA) or Akt, which may enhance or suppress PDE3A activity depending on cellular context.
This antibody is widely utilized in research to investigate PDE3A’s involvement in signaling pathways and disease mechanisms. For example, altered PDE3A activity has been linked to heart failure, diabetes, and obesity, making it a potential therapeutic target. By specifically recognizing the phosphorylated Ser312 epitope, the antibody enables studies of PDE3A activation states under conditions like hormonal stimulation, oxidative stress, or pathological remodeling. It is commonly applied in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess phosphorylation dynamics in cell lines, tissues, or preclinical models (e.g., mouse, rat). Researchers rely on its specificity to explore how PDE3A phosphorylation influences cyclic nucleotide signaling and contributes to disease progression or treatment responses.
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