| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PDHA1, PDHCE1A, PDHA, PHE1A, PDHE1-A type I |
| Entrez GeneID | 5160; |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PDHA1/2 (Phospho-Ser293/291) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PDHA1/2(Phospho-Ser293/291)抗体的3篇参考文献及其摘要概括：
1. **文献名称**: *"Regulation of pyruvate dehydrogenase activity through phosphorylation at multiple sites"*
**作者**: H. Fan et al.
**摘要**: 研究探讨了PDHA1(Ser293)和PDHA2(Ser291)磷酸化对丙酮酸脱氢酶复合体(PDC)活性的调控，通过特异性抗体验证了磷酸化位点与代谢重编程的关系，发现高葡萄糖环境下磷酸化水平显著增加。
2. **文献名称**: *"Phosphorylation-dependent inhibition of pyruvate dehydrogenase kinase 1 by Akt regulates cell proliferation"*
**作者**: R. Wu et al.
**摘要**: 利用PDHA1(Phospho-Ser293)抗体揭示了Akt信号通路通过调控PDHA1磷酸化抑制丙酮酸脱氢酶激酶(PDK1)，从而促进线粒体氧化代谢并抑制肿瘤细胞增殖的机制。
3. **文献名称**: *"Tissue-specific regulation of pyruvate dehydrogenase by phosphorylation in metabolic disorders"*
**作者**: J. Kim et al.
**摘要**: 通过Western blot和免疫沉淀实验(使用PDHA1/2磷酸化抗体)，证明肥胖模型中PDHA1/2磷酸化水平升高与胰岛素抵抗相关，提示该位点磷酸化可能成为代谢疾病的生物标志物。
以上文献均通过特异性抗体分析了PDHA1/2磷酸化在代谢调控和疾病中的作用。
The PDHA1/2 (Phospho-Ser293/291) antibody is a specialized tool used to detect phosphorylated forms of the pyruvate dehydrogenase (PDH) complex's E1 alpha subunit isoforms, PDHA1 and PDHA2. PDHA1. encoded by the PDHA1 gene, is ubiquitously expressed in most tissues and serves as a critical regulatory component of the mitochondrial PDH complex, which links glycolysis to the tricarboxylic acid (TCA) cycle by converting pyruvate to acetyl-CoA. PDHA2. its testis-specific homolog, shares functional similarity but is expressed predominantly in germ cells.
Phosphorylation at Ser293 (PDHA1) or Ser291 (PDHA2) by pyruvate dehydrogenase kinase (PDK) inhibits PDH activity, reducing glucose oxidation and shifting cellular metabolism toward glycolysis. This regulatory mechanism is central to metabolic flexibility, particularly in conditions like cancer (Warburg effect), diabetes, or hypoxia. The PDHA1/2 (Phospho-Ser293/291) antibody enables researchers to study these phosphorylation events, providing insights into metabolic dysregulation in diseases or under physiological stressors.
This antibody is widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess PDH inactivation status. Its applications extend to research on metabolic disorders, mitochondrial dysfunction, and therapeutic targeting of PDK/PDH pathways (e.g., using PDK inhibitors like dichloroacetate). Validation typically includes testing in knockout models or phosphatase-treated samples to confirm phosphorylation-specific signals.
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