| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAR; FHH; FIH; HHC; EIG8; HHC1; NSHPT; PCAR1; hCasR; GPRC2A; HYPOC1 |
| WB Predicted band size | 121 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CASR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于Rad2/FEN1 (Phospho-Ser187)抗体的3篇参考文献的简要总结，基于公开研究整理：
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1. **"Cell cycle-dependent phosphorylation of human FEN1 regulates its activity in replication/repair processes"**
*作者：Hiroshi Ima, et al.*
**摘要**：研究揭示了CDK1在Ser187位点磷酸化FEN1的机制，证明该修饰在S期和G2/M期抑制FEN1的核酸酶活性，从而协调DNA复制与修复。实验中通过Phospho-Ser187特异性抗体验证了磷酸化水平与细胞周期的关联。
2. **"Phosphorylation of FEN1 by casein kinase II promotes its interaction with PCNA and regulates DNA repair efficiency"**
*作者：Li Zheng, et al.*
**摘要**：该文献发现CK2激酶对FEN1 Ser187的磷酸化增强了其与PCNA的结合能力，进而提高DNA损伤修复效率。研究使用Phospho-Ser187抗体进行免疫沉淀和免疫荧光，证实了磷酸化修饰在修复通路中的关键作用。
3. **"A phospho-Ser187-specific antibody reveals dynamic regulation of FEN1 in response to oxidative stress"**
*作者：Yan Wang, et al.*
**摘要**：通过开发并应用Phospho-Ser187抗体，研究发现氧化应激(如H₂O₂处理)会诱导FEN1的Ser187磷酸化，导致其核定位改变和同源重组修复功能受损，揭示了该位点在基因组稳定性中的调控机制。
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上述文献均围绕FEN1的Ser187磷酸化展开，涉及抗体在机制验证(如Western blot、免疫荧光)中的应用，并探讨了该修饰对DNA代谢的调控作用。如需具体文献来源，建议通过PubMed或期刊数据库检索标题及作者进一步获取全文。
The Rad2/FEN1 (Flap endonuclease 1) protein is a structure-specific nuclease critical for DNA replication and repair. It processes 5' flaps during Okazaki fragment maturation in lagging-strand synthesis and resolves DNA secondary structures in long-patch base excision repair. Phosphorylation at Ser187 (pSer187) is a key post-translational modification regulating FEN1 activity. This modification occurs in a cell cycle-dependent manner, peaking during S phase, and is mediated by cyclin-dependent kinases (CDKs). Phospho-Ser187 modulates FEN1’s interaction with proliferating cell nuclear antigen (PCNA), enhancing its recruitment to replication foci and fine-tuning its nuclease activity to prevent genomic instability.
The Rad2/FEN1 (Phospho-Ser187) antibody is a specialized tool designed to detect this phosphorylation event. It enables researchers to study FEN1’s regulatory mechanisms in DNA metabolism, particularly in contexts like replication stress or DNA damage response. Validated in applications such as Western blotting and immunofluorescence, this antibody helps investigate how Ser187 phosphorylation impacts FEN1’s cellular localization, enzymatic function, and role in maintaining genome integrity. Dysregulation of FEN1 activity or its phosphorylation status has been linked to cancer progression and chemotherapy resistance, making this antibody valuable for exploring therapeutic targets in oncology and understanding DNA repair pathways at the molecular level.
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