关键信息
-
基因名
PRKAa2
- 应用
-
别名
PRKAa2；AMPK；AMPK2；5'-AMP-activated Protein kinase catalytic subunit alpha-2
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
O43741
-
表达区间
1-272aa
-
氨基酸序列
MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPI
-
分子量
57.3 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PRKAa2, a protein kinase belonging to the AMP-activated protein kinase (AMPK) family, has garnered significant attention in recent years due to its critical role in cellular energy homeostasis and metabolic regulation. As a central player in detecting changes in cellular energy levels, PRKAa2 is activated in response to increases in AMP or decreases in ATP, facilitating various downstream signaling pathways involved in energy production, glucose uptake, and lipid metabolism. Dysregulation of PRKAa2 signaling has been implicated in various metabolic disorders, including obesity, type 2 diabetes, and cardiovascular diseases. Recent studies suggest that modulating PRKAa2 activity could hold therapeutic potential for these conditions. The development of recombinant PRKAa2 proteins offers a unique opportunity to explore the biochemical properties and functional mechanisms of this kinase in detail. By producing and characterizing PRKAa2 in vitro, researchers can investigate its role in cellular signaling pathways, identify potential substrates, and assess the effects of pharmacological agents on its activity. Furthermore, the recombinant protein can be employed in structure-function studies to elucidate the molecular mechanisms underlying its regulation. Understanding PRKAa2's function may pave the way for novel therapeutic strategies targeting metabolic diseases, highlighting the importance of continued research in this area.
