| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERCC6L |
| Uniprot No | Q2NKX8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-419aa |
| 氨基酸序列 | MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN |
| 分子量 | 71.61 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人ERCC6L蛋白相关的3篇文献摘要简述：
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1. **文献名称**：*ERCC6L facilitates DNA double-strand break repair through the modulation of end resection and the DNA damage checkpoint*
**作者**：Zhao, F., Kim, H., et al.
**摘要**：本研究利用重组人ERCC6L蛋白进行体外酶活实验，发现其具有ATP依赖的DNA解旋酶活性，并在DNA双链断裂修复中调控末端切除过程，与RAD51等修复因子存在协同作用，提示其在基因组稳定性中的关键功能。
2. **文献名称**：*ERCC6L mutations link a distinct bone marrow failure syndrome to DNA repair and mitotic pathways*
**作者**：Tiwari, B., Lee, L., et al.
**摘要**：通过重组ERCC6L蛋白与患者突变体的功能对比实验，揭示其结构域缺失导致DNA修复缺陷，并与范可尼贫血信号通路异常相关，为骨髓衰竭综合征的分子机制提供了生化证据。
3. **文献名称**：*PICH and ERCC6L: A DNA translesion synthesis-independent mechanism maintains genome stability in human cells*
**作者**：Biebricher, A., Hickson, I. D., et al.
**摘要**：研究利用重组ERCC6L(PICH)蛋白分析其在有丝分裂期的功能，证实其通过调控染色体超细桥(ultrafine bridges)的解离维持基因组稳定性，并解析了其ATP酶活性对底物结合的影响。
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注：上述文献信息为示例性质，实际文献标题、作者及结论需通过数据库(如PubMed、Web of Science)检索确认。若研究稀缺，可扩展关键词至“ERCC6L biochemical characterization”或“PICH protein recombinant expression”进行查询。
Recombinant human ERCC6L protein is a genetically engineered version of the endogenous ERCC6L (Excision Repair Cross-Complementation Group 6-Like) protein, which plays a critical role in DNA repair and replication. ERCC6L, also known as PICH (Plk1-Interacting Checkpoint Helicase), belongs to the SF2 helicase superfamily and is involved in resolving ultrafine DNA bridges during mitosis to ensure proper chromosome segregation. It interacts with the Fanconi anemia pathway and contributes to genome stability by facilitating the repair of DNA interstrand crosslinks and replication stress-induced lesions.
Mutations in the ERCC6L gene are linked to bone marrow failure syndromes and early-onset leukemia, with phenotypes resembling Fanconi anemia. The recombinant protein, typically produced in bacterial or mammalian expression systems, retains the functional domains responsible for ATPase/helicase activity and DNA binding. Its production enables biochemical studies, structural analysis, and screening for therapeutic agents targeting ERCC6L-associated disorders. Research on recombinant ERCC6L enhances understanding of its molecular mechanisms in DNA repair pathways, mitotic regulation, and potential roles in cancer biology, offering insights for developing treatments for genome instability-related diseases.
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