| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERGIC2 |
| Uniprot No | Q96RQ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-215aa |
| 氨基酸序列 | MRRLNRKKTLSLVKELDAFPKVPESYVETSASGGTVSLIAFTTMALLTIMEFSVYQDTWMKYEYEVDKDFSSKLRINIDITVAMKCQYVGADVLDLAETMFASADGLVYEPTVFDLSPQQKEWQRMLQLIQSRLQEEHSLQDVIFKSAFKSTSTALPPREDDSSQSPNACRIHGHLYVNKVAGNFHITVGQFHILVVMHIWQHLSTMNLTIFLIE |
| 分子量 | 51 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ERGIC2蛋白的示例参考文献(注：部分文献为虚构示例，建议通过PubMed或Google Scholar查询真实文献)：
1. **文献名称**: "Characterization of Recombinant Human ERGIC2 and Its Role in ER-Golgi Trafficking"
**作者**: Li X, Wang Y, Zhang J
**摘要**: 研究利用哺乳动物表达系统表达重组人ERGIC2蛋白，发现其通过与COPII囊泡成分相互作用调控内质网-高尔基体间膜运输，并解析了其C端结构域在脂质结合中的关键作用。
2. **文献名称**: "Structural Insights into Human ERGIC2 Protein via Cryo-EM Analysis"
**作者**: Chen H, et al.
**摘要**: 通过冷冻电镜技术解析重组人ERGIC2的三维结构，揭示其与ERGIC1形成复合物的机制，提出其在早期分泌途径中作为膜运输支架的功能模型。
3. **文献名称**: "ERGIC2 Deficiency Disrupts Collagen Secretion and Causes Connective Tissue Disorders"
**作者**: Suzuki K, et al.
**摘要**: 利用CRISPR技术敲低ERGIC2的细胞模型显示，重组ERGIC2蛋白可挽救胶原蛋白运输缺陷，表明其在细胞外基质蛋白分泌中的关键作用，与遗传性结缔组织病相关。
4. **文献名称**: "Expression and Purification of Functional Human ERGIC2 in Escherichia coli"
**作者**: Gupta R, et al.
**摘要**: 首次报道在大肠杆菌中成功表达可溶性重组人ERGIC2蛋白，采用镍柱亲和层析纯化，并证实其通过体外实验与Sar1B GTP酶存在直接互作。
注：以上为模拟文献，建议通过学术数据库(如PubMed、Web of Science)以关键词“ERGIC2 recombinant”“human ERGIC2”检索真实研究。
The human ERGIC2 (Endoplasmic Reticulum-Golgi Intermediate Compartment 2) protein is a conserved, membrane-associated component of the ER-Golgi transport machinery. Located primarily in the ER-Golgi intermediate compartment (ERGIC) and cis-Golgi network, it facilitates protein trafficking between the endoplasmic reticulum (ER) and Golgi apparatus. Structurally, ERGIC2 contains a coiled-coil domain and interacts with COPII and COPI vesicle coat proteins, suggesting roles in vesicle budding, cargo sorting, and maintaining compartment integrity. Studies indicate it may act as a scaffold for assembly of transport complexes or regulate receptor recycling.
ERGIC2 is implicated in glycosylation, secretory pathway regulation, and stress responses. Dysregulation has been linked to diseases, including cancer (e.g., altered expression in hepatocellular carcinoma) and neurodegenerative disorders, possibly due to disrupted protein homeostasis. Recombinant ERGIC2 protein, typically produced in mammalian or insect expression systems with tags (e.g., His, GFP), enables functional studies of intracellular transport mechanisms, protein interactions, and disease-associated mutations. Current research focuses on its molecular interactions, role in autophagy, and potential as a therapeutic target for trafficking-related pathologies. Its conserved nature across eukaryotes underscores fundamental importance in cellular logistics.
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