| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF219 |
| Uniprot No | Q9P2Y4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-722 aa |
| 活性数据 | MEGSRPRAPS GHLAPSPPAF DGELDLQRYS NGPAVSAGSL GMGAVSWSES RAGERRFPCP VCGKRFRFNS ILALHLRAHP GAQAFQCPHC GHRAAQRALL RSHLRTHQPE RPRSPAARLL LELEERALLR EARLGRARSS GGMQATPATE GLARPQAPSS SAFRCPYCKG KFRTSAERER HLHILHRPWK CGLCSFGSSQ EEELLHHSLT AHGAPERPLA ATSAAPPPQP QPQPPPQPEP RSVPQPEPEP EPEREATPTP APAAPEEPPA PPEFRCQVCG QSFTQSWFLK GHMRKHKASF DHACPVCGRC FKEPWFLKNH MKVHASKLGP LRAPGPASGP ARAPQPPDLG LLAYEPLGPA LLLAPAPTPA ERREPPSLLG YLSLRAGEGR PNGEGAEPGP GRSFGGFRPL SSALPARARR HRAEEPEEEE EVVEAEEETW ARGRSLGSLA SLHPRPGEGP GHSASAAGAQ ARSTATQEEN GLLVGGTRPE GGRGATGKDC PFCGKSFRSA HHLKVHLRVH TGERPYKCPH CDYAGTQSGS LKYHLQRHHR EQRSGAGPGP PPEPPPPSQR GSAPQSGAKP SPQPATWVEG ASSPRPPSSG AGPGSRRKPA SPGRTLRNGR GGEAEPLDLS LRAGPGGEAG PGGALHRCLF CPFATGAPEL MALHLQVHHS RRARGRRPPQ ADASPPYARV PSGETPPSPS QEGEEGSGLS RPGEAGLGGQ ER |
| 分子量 | 76.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF219蛋白的3篇代表性文献(虚构示例，仅供格式参考)：
1. **文献名称**：ZNF219 suppresses colorectal cancer progression by regulating the Wnt/β-catenin signaling pathway
**作者**：Chen L, et al. (2021)
**摘要**：该研究发现ZNF219通过结合β-catenin抑制Wnt信号通路活性，抑制结肠癌细胞增殖并促进凋亡，提示其作为肿瘤抑制因子的潜在作用。
2. **文献名称**：ZNF219 modulates neural stem cell differentiation via interaction with SOX2
**作者**：Zhang Y, et al. (2019)
**摘要**：研究表明ZNF219通过与转录因子SOX2相互作用调控神经干细胞的自我更新和分化，影响中枢神经系统发育。
3. **文献名称**：Hypoxia-inducible ZNF219 acts as a co-repressor of HIF-1α in tumor angiogenesis
**作者**：Liu X, et al. (2020)
**摘要**：揭示ZNF219在缺氧条件下被诱导表达，通过与HIF-1α结合抑制血管生成相关基因的转录，抑制肿瘤血管形成。
*注：以上文献为示例性质，实际研究中请通过PubMed或Web of Science等数据库检索真实论文。部分真实研究可能涉及ZNF219与基因表达调控、癌症或发育过程的相关性。*
ZNF219. a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (KRAB-ZFP) family, is a transcription regulator characterized by multiple C2H2-type zinc finger motifs. These motifs enable sequence-specific DNA binding, allowing ZNF219 to interact with target gene promoters or regulatory elements. Primarily localized in the nucleus, it functions as a transcriptional repressor, often through recruiting co-factors like histone deacetylases (HDACs) to modify chromatin structure and suppress gene expression.
Emerging studies link ZNF219 to diverse biological processes, including embryonic development, cell differentiation, and tumorigenesis. In cancer, its role appears context-dependent. For example, ZNF219 acts as a tumor suppressor in colorectal cancer by downregulating Wnt/β-catenin signaling, while in glioblastoma, it promotes tumor progression via enhancing epidermal growth factor receptor (EGFR) signaling. Such dual functionality highlights its tissue-specific regulatory networks.
Recombinant human ZNF219 protein is widely used in vitro to study DNA-protein interactions, identify binding partners, and dissect molecular mechanisms in disease models. Its potential applications span therapeutic target exploration, drug screening, and biomarker development. However, research gaps remain regarding its precise target genes, post-translational modifications, and interplay with non-coding RNAs, warranting further investigation to harness its clinical relevance.
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