| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF221 |
| Uniprot No | Q9UK13 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-617 aa |
| 活性数据 | MISPSLELLH SGLCKFPEVE GKMTTFKEAV TFKDVAVVFT EEELGLLDPA QRKLYRDVML ENFRNLLSVG NQPFHQDTFH FLGKEKFWKM KTTSQREGNS GGKIQIEMET VPEAGPHEEW SCQQIWEQIA SDLTRSQNSI RNSSQFFKEG DVPCQIEARL SISHVQQKPY RCNECKQSFS DVSVFDLHQQ SHSGEKSHTC GECGKSFCYS PALHIHQRVH MGEKCYKCDV CGKEFNQSSH LQTHQRVHTG EKPFKCGQCG KGFHSRSALN VHCKLHTGEK PYNCEECGKA FIHDSQLQEH QRIHTGEKPF KCDICGKSFR VRSRLNRHSM VHTGEKAFRC DTCGKNFRQR SALNSHSMVH IEEKPYKCEQ CGKGFICRRD FCKHQMVHTG EKPYNCKECG KTFRWSSCLL NHQQVHSGQK SFKCEECGKG FYTNSRRSSH QRSHNGEKPY NCEECGKDYK RRLDLEFHQR VHTGERPYNC KECGKSFGWA SCLLKHQRLH SGEKPFKCEE CGKRFTQSSQ LHSHQTCHTG EKLYKCEQCE KGYNSKFNLD MHQRVHGGER PYNCKECGKS FGWASCLLKH QRLHSGEKPL KSGVWEEIYS EFTASFTSVS LCGRKAI |
| 分子量 | 71.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于锌指蛋白家族研究的常见主题，模拟的3篇关于重组人ZNF221蛋白的参考文献(请注意，这些文献为示例，实际引用需核实真实性和存在性)：
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1. **文献名称**: *Cloning, Expression, and Functional Analysis of Human Zinc Finger Protein ZNF221*
**作者**: Li, X., et al.
**摘要**: 该研究报道了人源ZNF221基因的克隆及其在大肠杆菌系统中的重组表达。通过亲和层析纯化获得了高纯度蛋白，并证实了其在体外与特定DNA序列的结合活性，暗示其可能在转录调控中发挥作用。
2. **文献名称**: *ZNF221 Interacts with Histone Deacetylases and Modulates Transcriptional Repression in Cancer Cells*
**作者**: Wang, Y., & Zhang, R.
**摘要**: 研究发现重组ZNF221蛋白与HDAC复合物存在相互作用。实验通过体外Pull-down和荧光素酶报告基因分析表明，ZNF221可能通过招募组蛋白去乙酰化酶参与肿瘤相关基因的表观遗传沉默。
3. **文献名称**: *Structural Characterization of Recombinant ZNF221 and Its Role in Myoblast Differentiation*
**作者**: Gupta, S., et al.
**摘要**: 本研究利用哺乳动物细胞系统表达了重组ZNF221蛋白，并通过X射线晶体学解析了其锌指结构域的三维结构。功能实验显示，ZNF221在肌肉细胞分化中可能通过调控MyoD等肌源性因子表达发挥作用。
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**提示**：以上文献为示例，实际研究中建议通过 **PubMed、Web of Science** 或 **Google Scholar** 搜索关键词 "ZNF221 recombinant protein" 或 "ZNF221 function" 获取真实文献。若研究较少，可扩展至锌指蛋白家族(如ZNF217/ZNF222等)的相关文献。
ZNF221 is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein (ZNF) family, which constitutes one of the largest transcription factor families in humans. These proteins are characterized by tandem arrays of C2H2-type zinc finger motifs that mediate sequence-specific DNA binding, enabling regulatory roles in gene expression. The KRAB domain, located at the N-terminus, typically recruits repressive chromatin modifiers, suggesting ZNF221 may function as a transcriptional repressor. While the precise biological roles of ZNF221 remain understudied, it is postulated to participate in developmental processes, cellular differentiation, and homeostasis, similar to other KRAB-ZNF proteins. Dysregulation of ZNF family members has been implicated in cancers, neurological disorders, and immune diseases, though direct links to ZNF221 require further exploration. Recombinant human ZNF221 protein is engineered for in vitro studies, enabling biochemical characterization, interaction assays (e.g., DNA/protein binding), and antibody production. Its recombinant form, often expressed in bacterial or mammalian systems with affinity tags, facilitates purification and functional analyses. Research on ZNF221 could uncover novel regulatory pathways or therapeutic targets, particularly in contexts of aberrant gene silencing or epigenetic dysregulation. Current efforts focus on mapping its DNA binding specificity, downstream targets, and cross-talk with other transcriptional networks.
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