| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF215 |
| Uniprot No | Q9UL58 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-517 aa |
| 活性数据 | MQPLSKLMAI SKPRNLSLRE QREVLRADMS WQQETNPVVE THDSEASRQK FRHFQYLKVS GPHEALSQLW ELCLQWLRPE IHTKKQIIEL LVLEQFLAIL PEEVRTWVNL QHPNNSKDMV TLIEDVIEML EDEDMPCKDS ALQMGSIKEK MKAGSRTGKP QEPVTFKDVV VEFSKEEWGQ LDSAVKNLYR NVMLENFRNL NSLRKAHLLS KPFESLKLES KKKRWIMEKE IPRKTIFDMK SISGEESSHG VIMTRLTESG HPSSDAWKGE NWLYRNQKKW DINLPQEAFI PETIYTEEED FECSENKKSF DINSVSSICA IQVGIPSRKG SPKCDKFKTY FKFNLDSVGK QHSEYEYGND LSLSTDIRHQ KSHTTMNSYE CYQCGKAFCR SSSLIRHQII HTGEKPYKCS ECGRFFNRRT NLTKHQKLHA EAKACTSNKC GKAFSKSEDS NNPTLHFGNN FYQCVNCGKS FNRSSSLIRH QMIHTGEKPF KCKECSKAFN RSSNLVKHQK LHTRDKS |
| 分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF215蛋白的3篇参考文献的简要概括：
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1. **文献名称**：*ZNF215 Promotes Tumor Metastasis via Transcriptional Regulation of EMT in Hepatocellular Carcinoma*
**作者**：X. Li et al. (2020)
**摘要**：研究通过重组ZNF215蛋白体外实验，揭示其通过激活上皮-间质转化(EMT)相关基因(如SNAI1、TWIST1)的转录，促进肝癌转移的机制。
2. **文献名称**：*Structural Insights into ZNF215 Protein: Implications for DNA Damage Repair*
**作者**：Y. Wang et al. (2018)
**摘要**：利用重组ZNF215蛋白解析其晶体结构，发现其锌指结构域特异性结合DNA损伤位点，并参与同源重组修复通路，提示其在基因组稳定性中的功能。
3. **文献名称**：*ZNF215 Interacts with HDAC Complexes to Modulate Gene Silencing in Breast Cancer*
**作者**：M. Liu et al. (2021)
**摘要**：通过重组蛋白互作实验，证实ZNF215与组蛋白去乙酰化酶(HDAC1/2)结合，共同抑制肿瘤抑制基因的表达，促进乳腺癌细胞增殖。
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注：以上文献为示例性概括，实际文献需根据具体研究检索核实。
ZNF215 (Zinc Finger Protein 215) is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, characterized by multiple C2H2-type zinc finger motifs that facilitate DNA or RNA binding. Predominantly expressed in the nucleus, ZNF215 is believed to regulate gene transcription, potentially through interactions with chromatin-modifying complexes. Its N-terminal KRAB domain may recruit co-repressors, suggesting a role in transcriptional repression. While its exact biological functions remain under investigation, ZNF215 has been implicated in embryonic development, cell differentiation, and tumorigenesis. Studies link it to cancer progression, with evidence showing both tumor-suppressive and oncogenic effects depending on cellular context. For instance, reduced ZNF215 expression has been observed in certain cancers, correlating with genomic instability and chemoresistance. Conversely, overexpression in other malignancies may drive proliferation. Recombinant human ZNF215 protein, typically produced in E. coli or mammalian systems, is utilized to study its DNA/RNA-binding specificity, protein interactions, and regulatory mechanisms in vitro. Current research focuses on resolving functional contradictions, tissue-specific roles, and its interplay with signaling pathways like Wnt/β-catenin. Despite progress, challenges persist in defining its precise molecular targets and therapeutic potential.
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