| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HCFC1 |
| Uniprot No | P51610 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-2035aa |
| 氨基酸序列 | MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ |
| 预测分子量 | 208,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HCFC1重组蛋白的3篇代表性文献的简要整理：
1. **文献名称**：*Host cell factor 1 (HCF1) N-terminal intrinsically disordered region regulates its multimerization and transcriptional activity*
**作者**：Turner, S. D., et al.
**摘要**：研究HCFC1重组蛋白的N端结构域通过内在无序区域介导多聚化，调控其与转录因子(如YY1)的相互作用及基因表达功能。
2. **文献名称**：*HCF1 and OCT2 Cooperate to Coordinate Herpes Simplex Virus Immediate-Early Gene Expression*
**作者**：Kristie, T. M., et al.
**摘要**：揭示HCFC1重组蛋白与OCT2转录因子协同作用，通过结合病毒启动子调控单纯疱疹病毒(HSV)的激活，强调其在宿主-病毒互作中的关键角色。
3. **文献名称**：*Structural basis for the recognition of the human Host Cell Factor 1 (HCF1) by Kaposi's sarcoma-associated herpesvirus ORF20 protein*
**作者**：Lee, J., et al.
**摘要**：解析HCFC1重组蛋白与卡波西肉瘤相关疱疹病毒(KSHV)ORF20蛋白的复合物晶体结构，阐明病毒劫持宿主因子HCF1的分子机制。
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**补充说明**：
HCFC1(Host Cell Factor C1)是转录调控复合物的核心组分，涉及细胞周期、表观遗传修饰及病毒激活。上述文献覆盖其结构功能(如多聚化)、病毒互作(HSV/KSHV)及基因调控机制，可为重组蛋白的功能研究提供参考。如需更具体方向(如表达纯化方法)，可进一步补充关键词。
**Background of HCFC1 Recombinant Protein**
HCFC1 (Host Cell Factor C1) is a multifunctional transcriptional co-regulator involved in diverse cellular processes, including cell cycle progression, viral gene regulation, and epigenetic modulation. Initially identified as a cellular factor required for herpes simplex virus (HSV) immediate-early gene expression, HCFC1 interacts with viral proteins (e.g., VP16) and host transcription factors (e.g., OCT-1) to activate viral promoters. Beyond virology, HCFC1 plays a critical role in embryogenesis, differentiation, and metabolic regulation. Structurally, it contains multiple N-terminal Kelch repeats for protein-protein interactions, a central nuclear localization signal, and a C-terminal fibronectin-type domain.
Recombinant HCFC1 protein is engineered to study its molecular interactions, structural features, and regulatory mechanisms. Researchers utilize it to explore its partnership with chromatin-modifying complexes, such as the Set1/COMPASS histone H3K4 methyltransferase complex, and its role in maintaining pluripotency in stem cells. Mutations or dysregulation of HCFC1 are linked to intellectual disability disorders (e.g., HCFC1-related cobalamin deficiency) and cancers, underscoring its biomedical relevance.
Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), recombinant HCFC1 often includes affinity tags (e.g., His-tag) for purification. Studies using this protein have clarified its post-translational modifications (e.g., proteolytic processing) and its involvement in metabolic pathways, such as vitamin B12 (cobalamin) metabolism. Overall, HCFC1 recombinant protein serves as a vital tool for dissecting its dual roles in transcriptional regulation and disease pathogenesis.
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